中国组织工程研究Issue(6)：841-846,6.DOI:10.3969/j.issn.2095-4344.2014.06.004
人骨髓间充质干细胞神经元样细胞分化中自噬相关基因Beclin-1的表达
Autophagy-related gene Beclin-1 expression in neuron-like differentiation of human bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Bone marrow mesenchymal stem cells have potential to self-renewal and multi-lineage differentiation. But after a long period of culture in vitro, the proliferation and differentiation capacities of bone marrow mesenchymal stem cells gradual y loss, the mechanism underlying which is not clear now. <br> OBJECTIVE:To observe the expression of autophagy-related gene Beclin-1 in differentiation from human bone marrow mesenchymal stem cells into neuron-like cells in vitro. <br> METHODS:The changes of morphological characteristics of neuron-like cells differentiated from human bone marrow mesenchymal stem cells induced by epidermal growth factor were observed. The expression of neuron-specific enolase and glial fibril ary acidic protein in treated and untreated human bone marrow mesenchymal stem cells were detected using immunocytochemistry. The Beclin-1 protein expressions were detected by western blot before and after induction. <br> RESULTS AND CONCLUSION:After being induced, human bone marrow mesenchymal stem cells presented classical neuron-like morphology;the expressions of neuron-specific enolase and glial fibril ary acidic protein were 78.7%and 8.1%, respectively. The expression of Beclin-1 protein was changed correspondingly during the induction, which increased after 30 minutes of induction and decreased gradual y after 1 hour of induction. Human bone marrow mesenchymal stem cells could be induced into neuron-like cells in vitro by epidermal growth factor. Autophagy-related gene was highly expressed in the induction of early differentiation and the expression gradual y reduced until it remained at a low level during the differentiation.关键词
干细胞/骨髓干细胞/人骨髓间充质干细胞/神经元样细胞分化/体外实验/表皮生长因子/自噬/Beclin-1/江苏省自然科学基金Key words
autophagy/celldifferentiation/mesenchymal stem cells/glial fibril ary acidic protein/neurons分类
医药卫生引用本文复制引用
杨翼,丁文静,董万利..人骨髓间充质干细胞神经元样细胞分化中自噬相关基因Beclin-1的表达[J].中国组织工程研究,2014,(6):841-846,6.基金项目
江苏省自然科学基金项目资助(SBK201123617)@@@@the Natural Science Foundation of Jiangsu Province, No. SBK201123617 （SBK201123617）
