中国组织工程研究Issue(7)：1051-1056,6.DOI:10.3969/j.issn.2095-4344.2014.07.012
胶原诱导性关节炎大鼠滑膜血管内皮生长因子与微血管密度
Correlation between the expression of vascular endothelial growth factor and microvessel density in collagen-induced arthritis rats
摘要
Abstract
BACKGROUND:Vascular endothelial growth factor is found the strongest to promote angiogenesis, which show strong expression in synovial cells in rheumatoid arthritis. <br> OBJECTIVE:To evaluate the relation between the expression of vascular endothelial growth factor and microvessel density in rheumatoid arthritis. <br> METHODS:Col agen II and complete Freund’s adjuvant were used to induce experimental arthritis in Sprague-Dawley rats. Synovium expression of vascular endothelium growth factor and microvessel density were assayed at different time points using immunohistochemistry method, and changes in new vessels, vascular endothelial growth factor expression and arthritis index were observed along the time course. The correlations between vascular endothelial growth factor expression and number of new blood vessels as wel as between arthritis index and vascular endothelial growth factor expression were studied with linear correlation analysis and rank correlation analysis, respectively. <br> RESULTS AND CONCLUSION:With the time lasting, new blood vessels in the synovium proliferated, the synovium was thickened, and the arthritis index rose gradual y as wel as vascular endothelial growth factor expression and number of new microvessels were on the rise. Vascular endothelial growth factor expression was positively correlated with arthritis index (r=0.535, P<0.05) and significantly positively correlated with number of new microvessels (r=0.860, P<0.01). Vascular endothelial growth factor is involved in the course of synovium pannus response, and vascular endothelial growth factor and microvessel density may play an important role in rheumatoid arthritis pathogenesis.关键词
组织构建/血管内皮细胞/类风湿关节炎/血管内皮生长因子/微血管密度/滑膜/大鼠/国家自然科学基金Key words
arthritis, rheumatoid/collagen/vascular endothelial growth factors/synovial membrane/microvessels分类
医药卫生引用本文复制引用
杨波,梁清华,吴丹,熊新贵..胶原诱导性关节炎大鼠滑膜血管内皮生长因子与微血管密度[J].中国组织工程研究,2014,(7):1051-1056,6.基金项目
国家教育部博士点新教师基金(20090162120064)；国家自然科学基金(30371833)；湖南省中医药管理局项目(210046)；中南大学青年教师助推项目(2011QNZT137)@@@@the Ph.D. Programs Foundation of Ministry of Education of China, No.20090162120064 （20090162120064）
the National Natural Science Foundation of China, No.30371833 （）
the Projects of Hunan Province Administration of Traditional Chinese Medicine, No.210046 （）
Young Teacher Boosting Project of Central South University, No.2011QNZT137 （）
