中国组织工程研究Issue(7)：1069-1075,7.DOI:10.3969/j.issn.2095-4344.2014.07.015
帕金森病大鼠模型学习记忆能力与左旋多巴的关系
Relationship between learning and memory capacities and levodopa in rat models of Parkinson’s disease
摘要
Abstract
BACKGROUND:Classical drug for Parkinson’s disease is levodopa, but long-term application of levodopa can induce complications such as dyskinesias. <br> OBJECTIVE:To observe the effects of levodopa on learning and memory capacities of Parkinson’s disease rats and to study its mechanisms. <br> METHODS:The rat models of Parkinson’s disease were established using 6-hydroxydopamine. The 228 model rats were randomly divided into control group and experimental group. Rats in the experimental group were intraperitoneal y injected with 10, 20 and 30 mg/(kg•d) levodopa for 28 consecutive days. At 1, 3, 5, 7, 14 and 28 days after intraperitoneal injection, we observed the rats’ learning and memory capacities and tested plasma concentration of homocysteine and folic acid. Acetylcholinesterase activities in the rat hippocampus were measured. Hippocampal neurofibril ary tangles were observed using Bielschowsky staining. <br> RESULTS AND CONCLUSION:Increased dose of levodopa and prolonged application time obviously decreased learning and memory capacities in rats (P<0.001), increased plasma homocysteine levels, reduced folic acid levels (P<0.001), diminished acetylcholine esterase activities in the rat hippocampus (P<0.001), and increased neurofibril ary tangles in the rat hippocampus (P=0.000). Results suggested that a large dose of levodopa could significantly decrease the learning and memory capacities, and disease acetylcholine esterase activities, and increase neurofibril ary tangles in hippocampus. Its mechanism possibly associated with the increased plasma concentration of homocysteine.关键词
组织构建/组织工程/左旋多巴/帕金森病/学习记忆/乙酰胆碱酯酶/神经原纤维缠结/同型半胱氨酸/大鼠Key words
levodopa/Parkinson’s disease/learning/acetylcholine esterase/homocysteine分类
医药卫生引用本文复制引用
邢红霞,袁燕,刘胜,殷闯,韩金红,周慧聪,苏洲,郭双喜,王玉梅..帕金森病大鼠模型学习记忆能力与左旋多巴的关系[J].中国组织工程研究,2014,(7):1069-1075,7.基金项目
2006年新乡医学院立项资助项目@@@@the Xinxiang Medical University Project in 2006 （）
