中国组织工程研究Issue(8)：1191-1197,7.DOI:10.3969/j.issn.2095-4344.2014.08.008
以交联透明质酸钠为支架体外构建组织工程软骨
Construction of tissue-engineered cartilage with cross-linked sodium hyaluronate as scaffold materials in vitro
摘要
Abstract
BACKGROUND:Tissue engineering provides new ideas and approaches for repair of cartilage defects. <br> OBJECTIVE:To develop a complete set of solutions for construction of tissue engineered cartilagein vitro, with chondrocytes as seed cels and cross-linked sodium hyaluronate as scaffold materials. <br> METHODS: New Zealand rabbit articular chondrocytes were isolated, counted, and then cultured and passaged to prepare cellsuspension. Toluidine blue staining, RT-PCR and immunocytochemistry were exerted to evaluate the cultured cels. Chondrocytes were seeded and co-cultured with cross-linked sodium hyaluronate scaffold for 21 days. Then, RNA was isolated for RT-PCR, and frozen sections were prepared for morphological observation and immunohistochemistry study. <br> RESULTS AND CONCLUSION:The chondrocytes could adhere to the cross-linked sodium hyaluronate scaffold and aggregate, growing between fibers or adhering to the scaffold in a monolayer manner. The transcripts of cartilage specific aggrecan gene and colagen type II alpha 1 gene and cartilage specific protein colagen type II were expressed in cel-scaffold complexes to maintain the phenotype of chondrocytes. Cel-scaffold complexes co-cultured in vitro can form cartilage extracelular matrix, by which tissue engineered cartilage is expected to be obtained.关键词
生物材料/软骨生物材料/组织工程/关节软骨/支架材料/软骨细胞/分离培养/免疫细胞化学/RT-PCR/形态学观察/交联透明质酸钠/体外Key words
biocompatible materials/chondrocytes/hyaluronic acid/cross-linking reagents分类
医药卫生引用本文复制引用
刘少英,陈建英,陈倩倩,刘飞,刘霞,朱希强,凌沛学..以交联透明质酸钠为支架体外构建组织工程软骨[J].中国组织工程研究,2014,(8):1191-1197,7.基金项目
山东省优秀中青年科学家科研奖励基金(BS2011YY050)；济南市高校院所自主创新计划(201202038)@@@@Promotional Research Fund for Excelent Young and Middle-Aged Scientists of Shandong Province, No. BS2011YY050 （BS2011YY050）
the Independent Innovation Plan in Universities of Shandong Province, No.201202038 （）
