中国组织工程研究Issue(8)：1205-1210,6.DOI:10.3969/j.issn.2095-4344.2014.08.010
反相高效液相色谱法测定壳聚糖纳米粒中丙酮酸乙酯的含量
Determination of ethyl pyruvate in chitosan nanoparticles by reversed-phase high-performance liquid chromatography
摘要
Abstract
BACKGROUND:Research on ethyl pyruvate detection methods is reported rarely, and moreover, literature about reversed-phase high-performance liquid chromatography (RP-HPLC) for detection of ethyl pyruvate is less. <br> OBJECTIVE:To establish an RP-HPLC method for determination of ethyl pyruvate in ethyl pyruvate-chitosan nanoparticles. <br> METHODS: The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm× 150 mm, 5μm) at 25℃, with the mixture of acetonitrile and water (40:60, V/V) as the mobile phase at the flow rate of 1 mL/min. The determination wavelength wasset at 210 nm and the injection volume was 20 μL. <br> RESULTS AND CONCLUSION: The peak of ethyl pyruvate and the peaks of auxiliary materials and solvent were separated wel. The linear rang of ethyl pyruvate was 1-100 mg/L (r=0.999 6). The relative standard deviation of both the intra-and inter-day precision was less than 3% for low-, moderate-, and high-concentration ethyl pyruvate. The relative standard deviation of reproducibility test and stability test was 1.25% and 1.3%, respectively. Sample average recovery rates were (91.5±1.0)%, (3.5±0.2)%, (94.4±0.4)%, respectively. Encapsulation efficiency of samples were (87.2±0.22)%, (90.5±0.15)%, (91.1±0.17)%, respectively. The relative standard deviation of different sample content were 0.9%, 0.5%, 0.3%, respectively. The RP-HPLC method for determination of ethyl pyruvate is sensitive, accurate and highly specific with wide linear range and high sample average recovery.关键词
生物材料/纳米材料/药物控释材料/壳聚糖纳米粒/丙酮酸乙酯/反相高效液相色谱/包封率/相对标准偏差Key words
biocompatible materials/chitosan/nanoparticles/pyruvic acid分类
医药卫生引用本文复制引用
徐凤娥,赵兵新,孙树茂,孟永春,南开辉,俞阿勇,瞿佳..反相高效液相色谱法测定壳聚糖纳米粒中丙酮酸乙酯的含量[J].中国组织工程研究,2014,(8):1205-1210,6.基金项目
浙江省重点科技创新团队计划资助项目(2009R50039，2011R09039-06)@@@@the Major Scientific Innovative Team Plan of Zhejiang Province, No.2009R50039,2011R09039-06 （2009R50039，2011R09039-06）
