中国组织工程研究Issue(10)：1621-1628,8.DOI:10.3969/j.issn.2095-4344.2014.10.023
细胞重编程前后基因组的动态稳定性
Reprogramme-induced genomic stability
摘要
Abstract
BACKGROUND:Some studies have shown that more copy number variations are present in early passage human induced pluripotent stem cells than later passage human human induced pluripotent stem cells, their parental somatic fibroblasts or human embryonic stem cells. OBJECTIVE:To investigate whether the reprogramming process itself compromises genomic stability and further explore the efficiency of induced pluripotent stem cellestablishment. METHODS:Using high-resolution Affymetrix CytoScan HD array, we compared copy number variations and loss of heterozygosity in early passage induced pluripotent stem cells with their fibroblast cellorigins from genetic epilepsy patients. RESULTS AND CONCLUSION:Compared with somatic fibroblasts from genetic epilepsy patient, there was no difference in the loss of heterozygosity between the two types of cells, but more copy number variations were present in early passage human induced pluripotent stem cells which were characterized as microduplication and involved oncogenic genes. Results demonstrate the dynamic nature of genomic abnormalities during reprogramming process and the necessity of frequent monitoring human induced pluripotent stem cells to assure their genomic stability and clinical safety.关键词
干细胞/诱导/诱导多能性干细胞/重编程/拷贝数变异/杂合性缺失/遗传性癫痫/国家自然科学基金Key words
induced pluripotent stem cells/DNA copy number variations/genomics/loss of heterozygosity/epilepsy分类
医药卫生引用本文复制引用
曹定娅,李洁亮,刘维强,何文茵,何文智,骆玉梅,范勇,孙筱放..细胞重编程前后基因组的动态稳定性[J].中国组织工程研究,2014,(10):1621-1628,8.基金项目
国家自然科学基金面上项目(31171229)；国家自然科学基金委员会-广东省人民政府自然科学联合基金(U1132005) （31171229）
