中国组织工程研究Issue(11)：1641-1646,6.DOI:10.3969/j.issn.2095-4344.2014.11.001
热打击诱导氧化应激介导神经元凋亡过程中核因子κB的活化
Activation of nuclear factor kappa B during heat stress-induced neuronal apoptosis
摘要
Abstract
BACKGROUND:Hyperpyrexia can induce a wide range of cel apoptosis in organisms, but no study has introduced the mechanism of heat stress-induced neuronal apoptosis. <br> OBJECTIVE:To observe the effect of nuclear factor kappa B (NF-κB) signal pathway on heat stress-induced neuronal apoptosis through reactive oxygen species. <br> METHODS:Heat stress model was established in the cel incubator. Heat stress group of cel s were incubated at 39,41,43℃for2hours,whilecontrolgroupofcelswereincubatedat37 ℃in5% CO2 for 2 hours. Apoptosis was analyzed by flow cytometry using Annexin V-FITC/PI staining. The expression levels of caspase-3 and p-NF-κB65 were determined by western blot analysis. The amounts of intracel ular reactive oxygen species were assayed by DCFH staining. In addition, the effect of MnTMPyP and PTDC on heat stress-induced apoptosis was also studied. <br> RESULTS AND CONCLUSION:39 ℃ heat stress had no impact on the apoptosis, 41 ℃ heat stress induced a smal amount of apoptosis (10.19%), and 43 ℃ heat stress triggered a large amount of apoptosis (43.02%). The expression of caspase-3 and p-NF-κB65 was increased, in a temperature-dependent manner. In addition, both MnTMPyP and PTDC significantly decreased the heat stress-induced apoptosis and expression of caspase-3 and p-NF-κB65. Experimental findings indicate that, the increase of intracel ular reactive oxygen species may induce neuronal apoptosis, and NF-κB participates in the heat stress-induced neuronal apoptosis as the intermedial signal pathway.关键词
组织构建/组织工程/热打击/神经元/细胞凋亡/核因子κB/活性氧/caspase-3/抗-核因子κB65蛋白/国家自然科学基金Key words
heat stroke/apoptosis/NF-kappa B分类
医药卫生引用本文复制引用
刘云松,邓旭斌,霍少芬,苏磊..热打击诱导氧化应激介导神经元凋亡过程中核因子κB的活化[J].中国组织工程研究,2014,(11):1641-1646,6.基金项目
国家自然科学基金(81071529，81101467)；全军十二·五重点课题(BWS12J018)@@@@the National Natural Science Foundation of China, No.81071529,81101467 （81071529，81101467）
Military Key Project during the Twelfth Five-Year Plan, No. BWS12J018 （）
