中国组织工程研究Issue(11)：1700-1705,6.DOI:10.3969/j.issn.2095-4344.2014.11.010
胆囊收缩素促坐骨神经再生的可能机制
Possible mechanisms of cholecystokinin promoting sciatic nerve regeneration
摘要
Abstract
BACKGROUND:Previous studies have found that cholecystokinin octapeptide (CCK-8) can promote the regeneration after sciatic nerve injury in rats, but the exact mechanism remains unclear. <br> OBJECTIVE:To screen effective indicators and analyze the mechanism of CCK-8 promoting sciatic nerve regeneration from the perspective of nerve growth factor and nerve regeneration microenvironment. <br> METHODS:Healthy Sprague-Dawley rats, for the preparation of unilateral sciatic nerve transection injury model, were randomly divided into two groups. In the CCK-8 group, the animal model received intraperitoneal injection of CCK-8 (8 nmol/kg) for consecutive 7 days, while the control group was injected with equal volume of normal saline. The nerve growth factor expression, inducible nitric oxide synthase in the spinal cord, serum superoxide dismutase activity and malondialdehyde concentration, as wel as apoptotic cel s in spinal cord were al detected. <br> RESULTS AND CONCLUSION:In the CCK-8 group, nerve growth factor expression was higher than that in the control group (P<0.01), while inducible nitric oxide synthase and the number of apoptotic cel s were lower (P<0.01), serum superoxide dismutase activity was higher but malondialdehyde concentrations was lower (P<0.01, 0.05). The mechanisms of CCK-8 promoting sciatic nerve regeneration include protecting neurons, anti-apoptosis, inhibiting inflammatory response, anti-NO and anti-oxidation, reducing malondialdehyde, and al eviating free radical damage, as wel as stimulating nerve growth factor expression and release.关键词
组织构建/组织工程/坐骨神经损伤/神经再生/胆囊收缩素/神经生长因子/诱导型一氧化氮合酶/超氧化物歧化酶/丙二醛/细胞凋亡Key words
sciatic nerve/cholecystokinin/apoptosis/nerve growth factor分类
医药卫生引用本文复制引用
陈宣煌,李荣议,张国栋,林海滨,吴献伟,林宇进,郑锋..胆囊收缩素促坐骨神经再生的可能机制[J].中国组织工程研究,2014,(11):1700-1705,6.基金项目
福建省教育厅A类科技面上项目(JA11212)；福建省莆田市医学科技课题计划2012S03(4)@@@@Class A Science and Technology General Program of Fujian Provincial Education Bureau, No. JA11212 （JA11212）
Medical Technology Plan of Putian City of Fujian Province, No.2012S03(4) （4）
