中国组织工程研究Issue(11)：1737-1742,6.DOI:10.3969/j.issn.2095-4344.2014.11.016
C57BL/6小鼠胚胎成纤维细胞生物学特性及饲养层制备
Biological properties of C57BL/6 mouse embryonic fibroblasts and preparation of feeder layers
摘要
Abstract
BACKGROUND:Kunming mouse embryonic fibroblasts are the most common feeder layers at present, and there are rare reports addressing C57BL/6 mouse embryonic fibroblasts as feeder layers. <br> OBJECTIVE:To separate and culture C57BL/6 mouse embryonic fibroblasts in vitro, and produce feeder layers to enlarge the resources of mouse embryonic fibroblasts. <br> METHODS:C57BL/6 mouse embryonic fibroblasts were isolated and cultured by trypsin digestion method in vitro. The biological characteristics and growth rule of the fibroblasts were investigated, then the feeder layers for the cel culture were produced. The growth of cel colonies on the prepared feeder layer was tested. <br> RESULTS AND CONCLUSION:C57BL/6 mouse embryonic fibroblasts grew wel with a large amount, by trypsin digestion method at different concentrations. There was no significance in the survival rate after cryopreservation for 1 week, 2 weeks, 1 month, 3 months and 6 months. The cel s were proliferative from the second to fifth passage and declined sharply after the sixth passage. The planted mouse embryonic fibroblasts feeder layers had a high activity within 3 days, but got a sharp decline after 4 days. So it is best to use C57BL/6 mouse embryonic fibroblast feeder layers within 3 days after they’re inactivated. C57BL/6 mouse embryonic fibroblast feeder layer can support embryonic stem cel s and induce pluripotent stem cel s to grow as Kunming mouse embryonic fibroblasts.关键词
组织构建/成纤维细胞/小鼠胚胎/C57BL/6小鼠/细胞增殖/细胞活力/饲养层Key words
mice/embryonic development/fibroblasts/cel proliferation/trophoblasts/stem cel s分类
医药卫生引用本文复制引用
李颖,龚亚飞,陈欣洁..C57BL/6小鼠胚胎成纤维细胞生物学特性及饲养层制备[J].中国组织工程研究,2014,(11):1737-1742,6.基金项目
广东省高等学校科技创新项目(2012KJCX0087)，人多精受精合子作为体细胞核移植受体的发育潜能研究@@@@Scientific and Technology Innovation Project of Universities in Guangdong, No.2012KJCX0087 （2012KJCX0087）
