现代医药卫生Issue(7)：961-962,965,3.DOI:10.3969/j.issn.1009-5519.2014.07.001
免疫抑制剂干扰联合瘤细胞接种构建肝癌移植瘤小鼠模型
The establishment of transplanted hepatocellular carcinoma by immunosuppressor interference and inoculation with tu-mor cells
摘要
Abstract
Objective To explore the optimum method of establishing hepatocarcinoma-bearing mice′s model of trans-plantation tumor. Methods Totally 160 BALB/c mice were selected and randomized into dexamethasone group ,hepatoma carci-noma cell injection group,dexamethasone and hepatoma carcinoma cell injection group and blank control group,40 cases in each group and sex in half. The dexamethasone group was with intraperitoneal injection of dexamethasone;the hepatoma carcinoma cell injection group was directly inoculated hepatoma carcinoma cell line H22 in liver three times at different times;the dexamethasone and hepatoma carcinoma cell injection group combined with the above two methods and the blank control group was injected with normal saline. One month later,the mice were killed by cervical dislocation to extract liver tissue,calculate the liver index and ob-serve the pathological changes of liver tissue after hematoxylin-eosin(HE) staining. Results The cancer was not found in dex-amethasone group and control blank group. The percentage of carcinogenesis in liver tissue injection group ,dexamethasone and hepatoma carcinoma cell injection group was 71.4%(20/28) and 91.2%(21/23) respectively. Conclusion Hepatocarcinoma-bearing mice′s model of transplantation tumor can be established by inoculating mice hepatoma carcinoma cell directly and interfer-ence of dexamethasone combined with cyclophosphamide ,moreover combination with dexamethasone interference can improve the success rate of modeling significantly.关键词
肝肿瘤/肿瘤,实验性/接种/免疫抑制剂/肿瘤移植/小鼠Key words
Liver neoplasm/Neoplasms,experimental/Vaccination/Immunosuppressive agents/Neoplasm transplantation/Mice引用本文复制引用
黄衍强,莫小强,赵丽娟,陈秉朴,彭素芬,孙芹,胡海萍,杜艺冰,韦荣显..免疫抑制剂干扰联合瘤细胞接种构建肝癌移植瘤小鼠模型[J].现代医药卫生,2014,(7):961-962,965,3.基金项目
2009年广西壮族自治区教育厅科研项目（200911MS186）；2012年大学生创新课题（QJCX201211）；2013年校地校企共建创新平台课题（桂教科研[2013]8号）。 （）
