新医学Issue(11)：743-747,5.DOI:10.3969/g.issn.0253-9802.2013.11.003
羊水、脐带和骨髓来源间充质干细胞对淋巴细胞增殖的抑制效果比较
Inhibitory effect of amniotic fluid-derived mesenchymal stem cells versus umbilical cord blood mesen-chymal stem cells and bone marrow Mesenchymal stem cells on lymphocyte proliferation
摘要
Abstract
Objective To compare the inhibitory effect of amniotic fluid-derived mesenchymal stem cells ( AF-MSC) , umbilical cord blood mesenchymal stem cells ( UC-MSC ) and bone marrow Mesenchymal stem cells ( BM-MSC) on phytohemagglutinin ( PHA) -induced lymphocyte proliferation.Methods Human mesenchymal stem cells from the second trimester amniotic fluid , umbilical cord and normal adult bone mar-row, flow cytometry were extracted and detected the surface molecules of MSC.Induce the MSCs differentiate into osteogenic like and adipogenic like cells in virto.AF-MSC, UC-MSC and BM-MSC were added to PHA-in-duced lymphocyte proliferation system at different ratio , compared inhibitory effect of three different sources-de-rived mesenchymal stem cells on lymphocyte proliferation.Results Mesenchymal stem cell-specific surface molecules CD29, CD70 and CD90 were expressed by isolated AF-MSC, UC-MSC and BM-MSC, respectively.However, hematopoietic stem cell surface molecule CD 45 was not expressed.In vitro cultures of AF-MSC, UC-MSC and BM-MSC on lymphocyte proliferation inhibition , when lymphocytes: mesenchymal stem cells 1: 1, 1: 10, 1: 50, 1: 100, the right lymphocyte proliferation inhibition rate AF-MSC strongest, BM-MSC wea-kest.Whenever the of ratio of lymphocytes and mesenchymal stem cells are 1: 1, 1: 10, 1: 50, 1: 100, the lymphocyte proliferation inhibition of AF-MSC is strongest , and BM-MSC is weakest.Conclusion AF-MSC, UC-MSC and BM-MSC can inhibit PHA-induced lymphocyte proliferation , in which the AF-MSC is the strongest and BM-MSC is the weakest.Further study indicates that the effect was associated with the number of ratio of MSC to LC.关键词
羊水/脐带/骨髓/间充质干细胞/免疫抑制Key words
Amniotic fluid/Umbilical cord/Bone marrow/Mesenchymal stem cells/Immune sup-pression引用本文复制引用
朱海鹏,彭亮,王培培,杜巍,殷思纯,李发武..羊水、脐带和骨髓来源间充质干细胞对淋巴细胞增殖的抑制效果比较[J].新医学,2013,(11):743-747,5.基金项目
东莞市医疗卫生单位科技计划项目 （）
