新医学Issue(2)：88-94,7.DOI:10.3969/g.issn.0253-9802.2014.02.004
Smad3磷酸化促进溃疡性结肠炎的黏膜修复
Smad3 phosphorylation contributes to the recovery of ulcerative colitis
摘要
Abstract
Objective To investigate the process of mucosal injury and repair of ulcerative colitis in mice and the role of p-Smad3 which is the key factor in transforming growth factor-β(TGF-β)signaling path-way.Methods Mice were exposed to 3%dextran sulfate sodium (DSS)for 5 days to induce acute ulcerative colitis,then followed with water for 4 weeks for mucosal repair.Mice were divided into six groups:control group that were allowed to drink only water,injury group that were exposed to 3%DSS for 5 days and the other four recovery groups which drunk water for 1 ,2,3,4 weeks after DSS removal,respectively.General health condition was recorded daily and histological examination was performed to analysis clinical and histopathologi-cal changes of DSS-induced colitis.Related markers of apoptosis and proliferation were detected.The expres-sion of TGF-βand p-Smad3 were measured to observe the change of TGF-βsignaling pathway during the muco-sal injury and repair process.Results Disease activity index of mice increased after DSS treatment and re-turned to normal at 2 weeks after DSS removal.The histological score significantly increased in injury group and began to decrease after 2 weeks of recovery.The apoptotic index rose to the maximal level in injury group and back to normal at 3 weeks after DSS withdrawal.However,the proliferation index reduced to the minimal level in injury group,then started to increase to the peak at 3 weeks.TGF-βexpression increased in all experimental groups,while the activation of Smad3 was inhibited in injury group,then began to be reactivated at 1 week af-ter DSS removal.Conclusion Smad3 phosphorylation promotes the repair of colonic mucosa of ulcerative coli-tis in mice.关键词
溃疡性结肠炎/修复/增殖/转化生长因子-β/Smad3Key words
Ulcerative colitis/Recovery/Proliferation/Transforming growth factor-β/Smad3引用本文复制引用
陈婷婷,郑丰平,谭嗣伟,陶金,吴斌..Smad3磷酸化促进溃疡性结肠炎的黏膜修复[J].新医学,2014,(2):88-94,7.基金项目
广东省自然基金面上项目（S2012010008795）；广东省自然基金重点项目（S2011020002348）；广东省科技计划项目 （）
