疑难病杂志Issue(4)：399-402,4.DOI:10.3969/j.issn.1671-6450.2014.04.023
辛二酰苯胺异羟肟酸对裸鼠人宫颈癌移植瘤的作用及其机制
Inhibition effects of SAHA on human cervical cancer cells in nude mice xenografts
付鑫鑫 1田志华 2郝钢华 2刘占稳 3刘金 1邢军1
作者信息
- 1. 063000 唐山，河北联合大学附属医院妇产科
- 2. 062552 任丘市华北石油总医院二部医院妇产科
- 3. 任丘市人民医院妇产科
- 折叠
摘要
Abstract
Objective To observe the tumor growth inhibition effect of SAHA to human cervical cancer transplanted in nude mice and to discuss the mechanism .Methods Cultured human cancer cell lines in nude mice inoculated to 20 dorsal roots of the right lower limb subcutaneous , 15 d after the tumor will become 18 mice were randomly divided into three groups with each included 6 mice:blank control group, SAHA1 group (25 mg/kg), SAHA2 group (50 mg/kg).Consecutive 4d in-traperitoneal injection of drugs , intermittent 7 d, and then continuously administered 4 d, 1 times/d.Observe the growth of cancer xenografts in nude mice in each group , the inhibition rate was calculated .Immunohistochemistry and Western blot a-nalysis was used to detect the expression of tumor in each group Bax and Bcl-2 protein.Results Tumor suppression in blank control group was 0, SAHA1 group was 22.44%, SAHA2 group was 35.52% , the two groups was statistically significant difference ( P <0.05).After treatment, SAHA2 group nude body mass were lower than the blank control group and SAHA 1 group ( P <0.05).After treatment, SAHA1 group SAHA2 tumor volume was less than the blank control group , and the SA-HA2 group was less than SAHA1 group( P <0.05).Compared with the blank control group , SAHA1 group and SAHA2 nude mice's tumors with low quality, and SAHA2 group lower than SAHA1 group, the differences were statistically significant (P <0.05);in immunohistochemistry test and protein blotting method , compared with blank control group , tumor tissue Bax protein in SAHA1 group and SAHA2 group were significantly increased , Bcl-2 protein were significantly lowered ( P <0.05).the changes in SAHA2 group were more obvious than SAHA1 group ( P <0.05).Conclusion SAHA can inhibit human cancer xenograft SiHa cell growth , Bax/Bcl-2 -related apoptosis is one of the tumor suppressor mechanism .关键词
辛二酰苯胺异羟肟酸/宫颈癌/裸鼠/Bcl-2/BaxKey words
Suberoylanilide hydroxamic acid/Cervical cancer/Nude mice/Bcl-2/Bax引用本文复制引用
付鑫鑫,田志华,郝钢华,刘占稳,刘金,邢军..辛二酰苯胺异羟肟酸对裸鼠人宫颈癌移植瘤的作用及其机制[J].疑难病杂志,2014,(4):399-402,4.
