亚热带植物科学Issue(3)：241-244,4.DOI:10.3969/j.issn.1009-7791.2013.03.012
香附子块茎试管苗繁殖
The Study on Tuber and Tube Seedlings Breeding of Cyperus rotundus
摘要
Abstract
With the method of tissue culture, the tubers of Cyperus rotundus were used as materials to do the research on tube seedlings’ propagation. The results demonstrated that 1/3MS with the matrix of cinder was the ideal medium for adventitious buds growth, and 1/2MS+ZT 0.6 mg/L+NAA 0.1 mg/L was the optimum medium for differentiation cultivation and subculture of growth buds. The ideal medium for rooting and miniature tuber cultivation was 1/2MS+ABT2 6.0 mg/L+IAA 0.4 mg/L. The transplanting survival rate of tube seedlings after 30 days’ training and 60 days’ training were 96% and 89.7%. The sprout growth rate of tubers was 99.8%. The planting survival rate of tube seedlings and miniature tubers were above 99%. The tube seedlings stable planted maintained all botany characteristics of the wild Cyperus rotundus.关键词
香附子/块茎/试管苗/微型块茎Key words
Cyperus rotundus/tuber/tube seedlings/miniature tuber分类
农业科技引用本文复制引用
贺文迪,孙婧靓,李德鑫,宁淑香,姜长阳..香附子块茎试管苗繁殖[J].亚热带植物科学,2013,(3):241-244,4.基金项目
辽宁省普通高等教育本科教学改革研究项目(201203041-4) （201203041-4）
