摘要
Abstract
Objective endoplasmic reticulum stress in the unfolded protein response in the pathogenesis of cataract Relations. Methods grouping results using dif erent concentrations of standard homocysteine assay. Among them, using the MTT assay for the detection of dif erent concentrations of drugs for the role of inhibition of cel proliferation rate calculation; apoptosis rate of use Hoechst33258 fluorescent staining method to detect implementation; For stimulation of reactive oxygen species in the cel s use reactive oxygen species fluorescent probe DCFH-DA detected achieved; cel s after stimulation of reduced glutathione content GSH detection kit detects implementation; using Western blot techniques for the expression of GRP78 and caspase-12, the cel s after stimulation, The experimental detection applications. Results Dif erent concentrations of cysteine likely to cause lens epithelial cel viability in a concentration dependent decrease in change. Secondly, according to the reactive oxygen species in the cel s of the experimental results showing a concentration-dependent increase of changes in the cel s of reduced glutathione content showed a decreasing variation, showing elevated expression of caspase-12's detection change for GRP78. Conclusion The high concentration of the endoplasmic reticulum stimulating factor for lens epithelial cel s has a stimulating ef ect, this stimulating ef ect, not only can lead to endoplasmic reticulum stress in lens epithelial cel s, but also can be caused by the unfolded protein response crystals epithelial apoptosis occurs, thus resulting in cataract.关键词
未折叠蛋白反应/晶体上皮细胞/凋亡/白内障/发病机制/探析Key words
the unfolded protein response/Lens epithelial cells/Apoptosis/cataracts/Pathogenesis/Analysis
