渔业现代化Issue(6)：46-51,6.DOI:10.3969/j.issn.1007-9580.2013.06.010
巴氏灭菌即食小龙虾特定腐败菌的16S rRNA鉴定
Molecular identification of specific spoilage organisms from ready-to-eat crayfish sterilized by pasteurization
摘要
Abstract
To examine the spoilage microflora from aluminium-foil-vacuum-packed ready-to-eat crayfish sterilized by conventional pasteurization , species identification was made by amplified ribosomal DNA restriction analysis ( ARDRA) together with 16S rRNA sequence analysis.At the same time, traditional methods of isolation , colony appearance observation and microscopic examination of the bacteria were also employed to confirm the identification .As the result, 82 typical culture-dependent strains were isolated from the ready-to-eat crayfish samples, including dominant species of Bacillus thuringiensis 70 strains (85.37%), Serratia marcescens 7 strains (8.54%) and minority group 5 strains (6.09%).The results indicated that bacillus is the specific spoilage organism ( SSO ) which is hard to be eliminated thoroughly through conventional pasteurization .Therefore, more efficient sterilization methods or targeted preservatives should be adopted to prolong the shelf life during the ready-to-eat crayfish processing and storage .关键词
小龙虾/巴氏灭菌/特定腐败菌(SSO)/16S rRNAKey words
crayfish/pasteurization/SSO/16S rRNA分类
农业科技引用本文复制引用
张永进,魏静,王伟,林琳,叶应旺,崔峰,赖年悦,姜绍通,陆剑锋..巴氏灭菌即食小龙虾特定腐败菌的16S rRNA鉴定[J].渔业现代化,2013,(6):46-51,6.基金项目
安徽省虾蟹类产业技术体系；安徽省115产业创新团队计划 （）
