中国动物检疫Issue(9)：50-55,6.
传染性法氏囊病毒免疫原基因在毕赤酵母中的高效分泌表达
High Performance Secretion and Expression of vvIBDV VP2 in Pichia pastoris
蓝胜芝 1徐素珍 1李宝臣 1曹玉飞 1赵艳敏 1金顺福1
作者信息
- 1. 浙江诺倍威生物技术有限公司，浙江杭州 310018
- 折叠
摘要
Abstract
The VP2 gene of vvIBDV was inserted into the Pichia pastoris expression vector of pPICZαA. Then the recombinant plasmid of pPICZαA VP2 was transformed into X-33 by electroporation. The multi-copy insertion trans-formants X-33-pPICZαA-VP2 were screened by PCR、phenotype and Zeocin-resistance . The expressed VP2 accumu-lated up to about 0.459mg/mL. SDS-PAGE、Western blot and animal experiment demonstrated that VP2 product has the similar bioactivity and antigenicity as the natural one.关键词
鸡传染性法氏囊病(IBD)/超强毒株VP2/巴斯德毕赤酵母/高效分泌表达Key words
Infectious bursal disease(IBD)/Very virulent virusVP2/Pichia pastoris/Efficient Secretion expression分类
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蓝胜芝,徐素珍,李宝臣,曹玉飞,赵艳敏,金顺福..传染性法氏囊病毒免疫原基因在毕赤酵母中的高效分泌表达[J].中国动物检疫,2013,(9):50-55,6.
