中国动物检疫Issue(10)：57-60,4.
空肠弯曲杆菌ERIC-PCR分型技术研究
Study on construction and application of ERIC-PCR method for typing Campylobacter jejuni
摘要
Abstract
Objective To generate an efifcient enterobacterial repetitive intergenic consensus sequence (ERIC-PCR) typing and analyze molecular type of different food-borne Campylobacter jejuni.Method Genomic DNA of Campylo-bacter jejuni reference strain ATCC33560 was used as the template for PCR. Target sequences in Campylobacter jejuni genomic DNA were amplified with the primers designed according to the reference. To obtain optimum fingerprint maps,template and primer concentration of the reaction system and annealing temperature of PCR,the factors to be optimized were designed in different concentration grads and other factors were ifxed. Then 20 Campylobacter jejuni isolates were classiifed by PCR.Result The optimal concentration of template DNA was 96ng/25mL,the concentration of primers was 0.8mmol/L each primer and annealing temperature of PCR was 40 ℃.The ERIC-PCR ifngerprint maps of different isolates were different. The ampliifcation products contained 2 to 13 bands in length were ranging between 160bp and 32000bp. The 20 Campylobacter jejuni isolates were classiifed into 14 types according to ifngerprint maps. Conclusion The optimum ERIC-PCR molecular classiifcation method of Campylobacter jejuni was established. ERIC-PCR system is an efficient method for identification,typing and tracking analysis. It will be a promising method in studying molecular epidemiology of. Campylobacter jejuni.关键词
空肠弯曲杆菌/ERIC-PCR/分型Key words
Campylobacter jejuni/ERIC-PCR/typing分类
农业科技引用本文复制引用
唐梦君,高玉时,张小燕,陆俊贤,施祖灏,唐修君,陈大伟..空肠弯曲杆菌ERIC-PCR分型技术研究[J].中国动物检疫,2013,(10):57-60,4.基金项目
江苏省自然科学基金（BK2010297）；江苏省家禽科学研究所博士基金（JQ201002）；扬州市社会发展项目 （）
