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猪繁殖与呼吸综合征病毒的分离及ORF5基因的序列分析OA

Isolation of Porcine Reproductive and Respiratory Syndrome Virus and its ORF5 Gene Sequence Analysis

中文摘要英文摘要

采集菏泽地区某猪场PRRS疑似病料,在Marc-145细胞上进行病毒分离,得到一株病毒HZ0622,通过免疫荧光试验初步鉴定分离病毒为PRRSV。对ORF5基因进行克隆和测序,并利用DNAStar软件将测定序列与国内外代表性毒株进行同源性比较,结果表明:分离株HZ0622 ORF5基因与美洲型代表毒株VR-2332的同源性为89.55%,与LV株同源性为61.17%,与中国高致病性PRRSV毒株JXA1、HEB1、SY0608、SD-4的同源性分…查看全部>>

Tissue samples were collected from pigs infected suspiciously by in a Heze farm,a virus strain(HZ0622) was isolated by Marc-145 cell culture,and was tentatively conifrmed as porcine reproductive and respiratory syndrome virus with indirect lfuorescent antibody test(IFA). ORF5 gene of HZ0622 was cloned and sequenced,and aligned to the representative PRRSV isolates with DNAStar software. The results showed that the ORF5 gene of HZ0622 was 89.55%sequence simila…查看全部>>

温海燕

菏泽学院制药工程系,山东菏泽 274015

农业科技

猪繁殖与呼吸综合征病毒分离ORF5基因序列分析

PRRSVIsolationORF5 geneSequence analysis

《中国动物检疫》 2013 (10)

66-69,4

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