摘要
Abstract
Tissue samples were collected from pigs infected suspiciously by in a Heze farm,a virus strain(HZ0622) was isolated by Marc-145 cell culture,and was tentatively conifrmed as porcine reproductive and respiratory syndrome virus with indirect lfuorescent antibody test(IFA). ORF5 gene of HZ0622 was cloned and sequenced,and aligned to the representative PRRSV isolates with DNAStar software. The results showed that the ORF5 gene of HZ0622 was 89.55%sequence similarity with the North American prototype VR-2332,61.17%similarity with LV,and 98.84%, 98.34%,99% and 99.34% similarity respectively with highly pathogenic PRRSV strains including JXA1,HEB1, SY0608 and SD-4. The phylogenetic tree indicated that HZ0622 belonged to the same branch with the highly pathogenic PRRSV,and shared a closer relationship with the SD-4. HZ0622 isolate belonged to the North American genotype and possibly a highly pathogenic PRRSV strain.关键词
猪繁殖与呼吸综合征病毒/分离/ORF5基因/序列分析Key words
PRRSV/Isolation/ORF5 gene/Sequence analysis分类
农业科技
