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两种方法检测牛地方流行性白血病的比较

王作佳 赵祥平 王乃福 张永年 王玉玲 刘亚峰 董志珍

中国动物检疫Issue(10):74-76,3.
中国动物检疫Issue(10):74-76,3.

两种方法检测牛地方流行性白血病的比较

Comparison of indirect ELISA and AGID for Detecting EBLV Antibody in the Serum Samples Dairy Cattle

王作佳 1赵祥平 1王乃福 1张永年 1王玉玲 1刘亚峰 1董志珍1

作者信息

  • 1. 天津出入境检验检疫局,天津 300461
  • 折叠

摘要

Abstract

Objective to analyze the consistency of ELISA and AGID for detecting BLV antibody. Method The 3998 Se-rum samples of cows imported from Uruguay were collected and tested for the antibodies against BLV using ELISA and AGID. Result The results showed that the positive coincidence rate of the two methods was 100%(18/18),the nega-tive coincidence was 99.42%(3975/3998) and the total coincidence rate was 99.87%(3993/3998). Conclusion Both ELISA and AGID could be applied in diagnosis and serological screening of EBL,and the ELISA positive rate was higher than that of AGID method.

关键词

酶联免疫吸附试验(ELISA)/琼脂凝胶免疫扩散试验(AGID)/牛白血病病毒(BLV)

Key words

ELISA/AGID/BLV

分类

农业科技

引用本文复制引用

王作佳,赵祥平,王乃福,张永年,王玉玲,刘亚峰,董志珍..两种方法检测牛地方流行性白血病的比较[J].中国动物检疫,2013,(10):74-76,3.

基金项目

天津检验检疫科技技术项目 ()

中国动物检疫

1005-944X

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