中国动物检疫Issue(11)：71-74,4.
鉴别猪瘟病毒强毒和疫苗弱毒的RT-PCR检测方法的建立
A Reverse Transcription Polymerase Chain Reaction for Detection and Differentiation of Wild-type and Vaccine Strains of Classical Swine Fever Virus
摘要
Abstract
A reverse transcription polymerase chain reaction (RT-PCR) was developed for the differentiation of wild-type classical swine fever virus from the C-strain vaccine with primers specific respectively for CSFV C-strain vaccine and highly virulent Shimen strain as the forward primer and a common downstream primer designed according to the highly conserved regions of CSFV genome in GenBank. By this method, a fragment of 785bp was amplified from genomic RNA of C-strain and a fragment of 680bp was amplified from genomic RNA of Shimen strain. No amplification was achieved for genomic RNAs of PRRSV、BVDV、PRV、PCV etc. The method could detect 0.5pg of CSFV RNA. The study laid the foundation for the establishment of the laboratory tests and epidemiological investigation of CSFV.关键词
猪瘟病毒/强弱毒鉴别/RT-PCRKey words
Classical swine fever virus (CSFV)/Differentiation of virulent and avirulent virus strains/Reverse transcription polymerase chain(RT-PCR)分类
农业科技引用本文复制引用
陈本龙,张立怀,王建华,王乃福..鉴别猪瘟病毒强毒和疫苗弱毒的RT-PCR检测方法的建立[J].中国动物检疫,2013,(11):71-74,4.基金项目
天津检验检疫局科技项目 （）
