中国肺癌杂志Issue(3)：226-232,7.DOI:10.3779/j.issn.1009-3419.2014.03.08
525例肺癌中ALK阳性病例临床病理特征研究及检测方法探讨
The Research of Clinical Pathological Features of ALK Positive Lung Cancer in 525 Patients and the Discussion of Detection Methods
朱翔 1刘岩 1王华 1张燕 1陈剑 1张波 1李红威 2曹宝山 3柳晨 4梁莉 3王玉湘 1由江峰 1高菲 5马晓龙1
作者信息
- 1. 100191北京，北京大学医学部病理学系/北京大学第三医院病理科
- 2. 100022北京，北京市垂杨柳医院
- 3. 100191北京，北京大学第三医院肿瘤化疗与放疗病科
- 4. 100191北京，北京大学第三医院放射科
- 5. 100053北京，基因科技 上海 有限公司
- 折叠
摘要
Abstract
Background and objective hTe fusion (rearrangement) of anaplastic lymphoma kinase (ALK) gene has been identiifed as an import factor to the tumorigenesis and development of lung cancer. ALK tyrosine kinase inhibitors (ALK-TKIs) have been proved to have good effects to ALK positive lung cancers. hTe increasement of the relevance ratio of ALK will be very important to patients. hTe aim of this study is to investigate the clinical pathological features of ALK positive lung cancer, and the roles of immunohistochemistry (IHC) and lfuorescence in situ hybridization (FISH) in screening and conifrm-ing the ALK positive cases in the testing lfow of ALK. Methods IHC analysis of ALK in tumor specimens was performed on 525 lung cancer patients. 34 cases among them were conifrmed by FISH. Results hTe positive incidence of ALK by IHC was 5.14%(27/525). hTe ALK positive patients were signiifcantly younger than ALK negative patients (P<0.05), and femal was predominant (P<0.05). hTe proportion of solid predominant adenocarcinoma was signiifcantly higher in ALK positive patients (P<0.05). While acinar and lepidic predominant adenocarcinoma were signiifcantly lower in ALK positive patients (P<0.05). FISH was applied in 34 cases. hTe coincidence rate was increased with the increasement of positive intensity of IHC staining. All the IHC positive cases with or without EGFR mutation must be conifrmed by FISH. Conclusion IHC is a reliable detec-tion method to screening the ALK in lung cancer, and then enhance the relevance ration. To make a deifnite diagnosis of ALK positive lung cancer, FISH is a signiifcant detection method.关键词
肺肿瘤/ALK/EGFR/免疫组化/FISHKey words
Lung neoplasms/Anaplastic lymphoma kinase gene (ALK)/Epidermal growth factor receptor (EGFR)/Immunohistochemistry (IHC)/Fluorescence in situ hybridization (FISH)引用本文复制引用
朱翔,刘岩,王华,张燕,陈剑,张波,李红威,曹宝山,柳晨,梁莉,王玉湘,由江峰,高菲,马晓龙..525例肺癌中ALK阳性病例临床病理特征研究及检测方法探讨[J].中国肺癌杂志,2014,(3):226-232,7.
