中国骨伤Issue(3)：237-239,3.DOI:10.3969/j.issn.1003-0034.2014.03.016
人脱细胞半月板的制备
Preparation of human meniscus acellular matrix
周预 1刘玉杰 2黄靖香 2王瑞 1臧磊 1海涌1
作者信息
- 1. 首都医科大学附属北京朝阳医院骨科，北京 100043
- 2. 解放军总医院骨科，北京 100853
- 折叠
摘要
Abstract
Objective:To investigate development of a cell extraction process for preparing human meniscus acellular ma-trix,and morphology and biomechanical properties. Methods:Human meniscus were subjected to modified eight step deter-gent,then,the specimens were assessed by staining with haematoxylin eosin,toluidine blue,sirius red,saffron O,alcain blue and hoechst-33258,et al. The ultrastructure of the specimens was observed with scanning electron microscope. Transient re-covery rate of deformation,maximal recovery rate of deformation and maximal compressive strength were tested to determine the biomechanical properties of the scaffold. Results:Every stain confirmed that the celluar constituents of the specimens were removed. The specimens stained positively by staining with sirius red. Lacuna were found irregularly not only on the surface of the meniscus,but also in the meniscus with scanning electron microscope. Pores in the specinmens were large ,the diameter of pores was 80 to 760μm,porosity was over 67%. The transient recovery rate of deformation was (89.62±1.04)%,the maximal recovery rate of deformation was 100%and the maximal compressive strength was (3.04±0.13)N,when the specimens were compressed 30%. Conclusion:The modified eight step detergent can remove the immunogenic cell components from human meniscus,in addition,3D extracellular matrix can be retained. The scaffold has good biomechanical properties. This scaffold stands a good chance to be an implant for future tissue engineering of the human meniscus.关键词
人类/半月板,胫骨/脱细胞基质Key words
Humans/Menisci,tibia/Decellularization matrix引用本文复制引用
周预,刘玉杰,黄靖香,王瑞,臧磊,海涌..人脱细胞半月板的制备[J].中国骨伤,2014,(3):237-239,3.
