局解手术学杂志Issue(6)：591-593,3.DOI:10.11659/jjssx.1672-5042.201306004
构建hTNF-α/293基因细胞对人结肠癌细胞增殖影响的实验研究
Effect of human tumor necrosis factor alpha-sercreting on proliferation of LOVO colon cancer cells
摘要
Abstract
objective To observe the effects of co-culture of hTNFα-sercreting and human colon cancer cells LOVO on the proliferation of cancer cells. Methods The stable transfected hTNF-α/293 , mRNA of Hek-293 cell and protein expression were detected by RT-PCR and ELISA. The positive group was added hTNF-αfactor,and MTT assay was applied under the optical density 490 nm. Through human tumor cell proliferation inhibition experiment,the inhibitory effects on colon cancer cells ( LOVO) proliferation were observed. Results hTNF-α/293 cells and hTNF-α-positive group showed a significant lower A,which suggested that hTNF-α/293 cells and hTNF-α-positive group had significant inhibition on the proliferation of colon cancer cells. Conclusion The inhibition of hTNF-αsecreted by hTNF-α/293 cells on co-lon cancer cell proliferation shows significant dose-effect dependency,and hTNF-αexpresses a considerable inhibition on the colon cancer cell proliferation as positive drug.关键词
人结肠癌细胞LOVO/人肿瘤坏死因子α/人胚胎肾细胞Hek-293Key words
LOVO cells/human tumor necrosis factor-α/human embryonic kidney cell分类
医药卫生引用本文复制引用
高宇红,薛毅珑,潘静坤..构建hTNF-α/293基因细胞对人结肠癌细胞增殖影响的实验研究[J].局解手术学杂志,2013,(6):591-593,3.基金项目
十二五国家技术研究发展计划(863计划)基金项目(2012AA020808)(2012-2015) （863计划）
