中国康复理论与实践Issue(3)：211-214,4.DOI:10.3969/j.issn.1006-9771.2014.03.003
低频电针对脊神经结扎大鼠痛敏化的干预作用
Effect of Electroacupuncture with Low Frequency on Pain Sensitization of Rats Suffered from Spinal Nerve Ligation
摘要
Abstract
Objective To investigate the effect of electroacupuncture with low frequency on pain sensitization of neuropathic pain rats, and the possible mechanism of regulating peripheral pain sensitization. Methods Rat neuropathic pain model was established with the right L5 spinal nerve ligation. Zusanli (ST36) and Kunlun (BL60) were selected for electroacupuncture intervention. Mechanical allodynia, and the levels of stransient receptor potential vanilloid type 1 (TRPV1) and substance P (SP) in ipsilateral L5 and L4 dorsal root ganglion (DRG) were tested. A further validation experiment with TRPV1 agonist 6'-IRTX was carried out. Results Rats suffered from L5 spinal nerve liga-tion showed obvious pain sensitization. The levels of TRPV1 in ipsilateral L4 DRG and SP in ipsilateral L5 DRG were significantly elevated. Electroacupuncture with low frequency significantly relieved pain sensitization, and suppressed TRPV1 and SP increase. 6'-IRTX intraperito-neally administered significantly counteracted the anti-pain sensitization action of electroacupuncture with low frequency. Conclusion Elec-troacupuncture with low frequency is effective on neuropathic pain by intervening pain sensitization, which may be related to the regulation of DRG TRPV1 and SP.关键词
神经病理痛/电针/背根神经节/辣椒素受体/P物质Key words
neuropathic pain/electroacupuncture/dorsal root ganglion/transient receptor potential vanilloid type l/substance P分类
医药卫生引用本文复制引用
蒋永亮,尹小虎,沈亚芳,赵晓芸,何晓芬,方剑乔..低频电针对脊神经结扎大鼠痛敏化的干预作用[J].中国康复理论与实践,2014,(3):211-214,4.基金项目
1.国家自然科学基金项目(No.81072855)；2.浙江省自然科学基金项目(No.LY12H27015 （No.81072855）
No.Z2100979)。 （）
