中国康复理论与实践Issue(3)：215-220,6.DOI:10.3969/j.issn.1006-9771.2014.03.004
电针对兔腰肌急性钝挫伤后组织修复与碱性成纤维细胞生长因子/细胞外信号调节激酶信号通路的影响
Effects of Electroacupuncture on Morphology Repairment and Basic Fibroblast Growth Factor/Extracellular Signal-regulated Ki-nase Signal Pathway of Rabbits with Acute Lumbar Muscle Contusion
摘要
Abstract
Objective To observe the effect of electroacupuncture on basic fibroblast growth factor (bFGF), Desmin and extracellular signal-regulated kinase (ERK1/2) expression of rabbits with acute lumbar muscle contusion, and discuss the possible mechanism of muscle tissue regeneration and repair. Methods 40 male New Zealand rabbits were randomly divided into blank group (BG, n=10), model group (MG, n=10), Weizhong (BL40) electroacupuncture group (WG, n=10) and local electroacupuncture group (LG, n=10). Lumbar muscle inju-ry was established with blunt trauma, and the rabbits were assessed with Appearance Score. The WG and LG accepted electroacupuncture for 2 weeks after modeling. The lumbar muscle of each animal was stained with HE to observe pathological changes, and immunohistochem-ical staining was used to observe bFGF expression, and Western blotting was used to detect the expression of Desmin and ERK1/2 protein. Results Appearance Score was more in the MG, WG and LG than in the BG (P<0.01). Histological score ranked from more to less as MG, LG, WG and BG (P<0.05). The expression of bFGF ranked as LG, WG, MG and BG (P<0.01), Desmin ranked as WG=LG and BG=MG (P<0.05), ERK1/2 as LG=WG, MG and BG (P<0.05). Conclusion Electroacupuncture could promote the regeneration and repair of tissue after acute lumbar muscle contusion, which may be related to the up-regulation of bFGF/ERK signaling pathway.关键词
腰肌急性钝挫伤/电针/委中/碱性成纤维细胞生长因子/结蛋白/细胞外信号调节激酶/兔Key words
lumbar acute contusion/electroacupuncture/BL40/basic fibroblast growth factor/Desmin/extracellular signal-regulated protein kinase/rabbits分类
医药卫生引用本文复制引用
陈欢,彭博,李富运,于雪,卢虎英,卫肖艳,彭园园,范伊凡,张莉..电针对兔腰肌急性钝挫伤后组织修复与碱性成纤维细胞生长因子/细胞外信号调节激酶信号通路的影响[J].中国康复理论与实践,2014,(3):215-220,6.基金项目
1.国家自然科学基金项目(No.81141120)；2.高等学校博士学科点专项科研基金项目(No.20110013110014)。 （No.81141120）
