中国口腔种植学杂志Issue(1)：1-5,9,6.
不同浓度尼古丁对种植体骨愈合的影响
The effect of various concentration of nicotine on the osseointagration after implantation
孙鑫 1侯玉东 1滕腾 1刘顺振 1薛鹏飞1
作者信息
摘要
Abstract
Objective: To study the healing conditions of the soft and bone tissue around the implant and the expression of the OPG and BMP-2 under intervened by different concentration of nicotine. Methods:Twenty four SD rats were equally randomly applied for High-dose group, Middle-dose group, Low-dose group and Control group, and intervened with 2mg/kg/bid, 0.2mg/kg/bid, 0.02mg/kg/bid and saline for 2 weeks. Then the two tibia of the rats were implanted by the titanium implant with the surface acid-sandblasting process under continuous nicotine-intervened. The specimens were observed by X-ray, CT, qRT-PCR and SEM ( scanning electron microscope) at the end of 2nd and 4th weeks. Datum analyzed with SPSS17.0 One-Way ANOVA test and comparison in group analyzed with T test. Result:The X-ray shows that there is no significant difference in group, except the HDN and control group. The CT images and BMD show that the nicotine groups are significant lower than control group, and there is significant difference in nicotine groups. The qRT-PCR show that the expression of the BMP-2 and OPG is gradual reduced as the increasing dose of nicotine during the two weeks, but there is no difference between the LDN and the control group (1/0.93±0.17), and the genes expression of the groups are sharp reduced in the later time. The SEM shows that the trabecular bone is inhibited and the woven bone is becoming, the bone matrix is distribution and disordered arrangement as the level and the intervened time of nicotine increased. Conclusion: There is great relationship between the inhibition of osseointagration of peri-implant and the down-regulate the expression of osteoinductive activity genes, and the effect has obvious time and concentration dependence.关键词
尼古丁/种植体/浓度依赖性/骨保护素/骨形成蛋白2Key words
nicotine/implant/concentration dependence/OPG/BMP-2分类
医药卫生引用本文复制引用
孙鑫,侯玉东,滕腾,刘顺振,薛鹏飞..不同浓度尼古丁对种植体骨愈合的影响[J].中国口腔种植学杂志,2014,(1):1-5,9,6.
