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松塔景天组织培养与快速繁殖的研究

胡颖慧 马春祥 梁孝莉

中国林副特产Issue(2):3-6,4.
中国林副特产Issue(2):3-6,4.

松塔景天组织培养与快速繁殖的研究

Tissue Culture and Rapid Propagation of Sedum nicaeense

胡颖慧 1马春祥 1梁孝莉2

作者信息

  • 1. 黑龙江省农业科学院牡丹江分院,黑龙江 牡丹江 157041
  • 2. 牡丹江市种子管理处
  • 折叠

摘要

Abstract

The leaves were used as the explants and taking MS as the basic medium supplemented some different plant grow th regulators to establish the tissue culture and rapid propagation sys-tem of Sedum nicaeense All .through initial induction ,subculture proliferation ,rooting cultured and transplantation .The result showed that the suitable medium for callus was MS supplemented with 0 .5mg/L 6-BA and 0 .05mg/L NAA ,and the induction rate of callus reached 87 .78% ;The suitable medium for shoot induction was MS supplemented with 1 .0 mg/L 6-BA and 0 .1 mg/L NAA ,and the induction rate of shoot reached 88 .89% ;The optimal medium for roots induce-ment was 1/2MS supplemented with 0 .3mg/L IBA and the induction rate of roots reached 91.33% ;T he survival ratio of plantlets reached 84% .

关键词

松塔景天/组织培养/植物生长调节剂

Key words

Sedum nicaeense All ./Tissue culture/Plant grow th regulators

引用本文复制引用

胡颖慧,马春祥,梁孝莉..松塔景天组织培养与快速繁殖的研究[J].中国林副特产,2014,(2):3-6,4.

中国林副特产

1001-6902

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