中国麻业科学Issue(10)：221-225,5.
红麻雄性不育相关基因 atp1的表达初步分析
Preliminary Analysis of Hibiscus cannabinus atp1 Gene Correlated with Male Sterility
黄思齐 1陈艳翠 1李建军 1唐慧娟 1陈安国 1李德芳1
作者信息
- 1. 中国农业科学院麻类研究所，湖南长沙410205
- 折叠
摘要
Abstract
Specific primers of CDS were designed according to CDS region predicted.The atp1 genes were amplified using DNA and cDNA as template in kenaf male sterile line and its maintainer line respec-tively.The sequences of atp1 gene amplified from DNA and cDNA had no difference between kenaf male sterile line and its maintainer line by sequencing and sequence alignment.It suggested that the sterility was not caused by base mutation of atp1 gene.Semi-RT-PCR analysis indicated that atp1 gene ex-pressed in root, stem, leaf, petal, anther and pistil, but the expression level in leaf and anther of male sterile line was lower than maintainer line , which suggested that the male sterility in kenaf was possibly related to the expression decrease of atp1 gene.关键词
红麻/质核互作雄性不育/功能分析Key words
kenaf/cytoplasmic-nuclear male sterility/gene analysis分类
农业科技引用本文复制引用
黄思齐,陈艳翠,李建军,唐慧娟,陈安国,李德芳..红麻雄性不育相关基因 atp1的表达初步分析[J].中国麻业科学,2013,(10):221-225,5.
