中国麻业科学Issue(2)：57-63,7.
苎麻胞液型谷氨酰胺合成酶基因的克隆和超量表达载体构建
Cloning and Construction of Over -expression Vector of GS1 Gene from Ramie (Boehmeria nivea Gaud)
摘要
Abstract
The glutamine synthetase (GS) incluing cytosolic (GS1) and plastid (GS2) is an essen-tial enzyme in nitrogen metabolism pathway of higher plants.We reported the first cloning of cytosolic glutamine synthetase gene BnGS1-1 from ramie ( Boehmeria nivea Gaud) .The cDNA of BnGS1-1 with length of 1205 bp encoded GS1 polypeptide of 356 amino acids.Sequence and structural analyses showed that BnGS1-1 protein contained beta -Grasp and catalytic functional domains that were conser-vative with other plants GS.The pI and Mw was 5.64 and 39.19 KDa, the residues in site 56, 92, 249 and 297 was Asp, Cys, His and Glu respectively.The amino acid sequences derived from BnGS1-1 had extensive homology with GS1s from other higher plants.The phylogenetic tree displayed that the BnGS1-1 and GS1 from Glycine max, Phaseolus vulgaris, Medicago sativa and Gossypium raimondii in-corporated into the same sub -clade.In addition , the plant over -expression vector was successfully constructed according to homologous recombination technology.Therefore, our work provided material and theoretical basis for understanding and improving ramie forage quality.关键词
苎麻/GS1/基因克隆/超量表达载体/构建Key words
Boehmeria nivea Gaud/GS1/gene cloning/over-expression vector/construction分类
农业科技引用本文复制引用
郑建树,喻春明,陈平,王延周,谭龙涛,朱涛涛,陈继康,卢凌霄,熊和平..苎麻胞液型谷氨酰胺合成酶基因的克隆和超量表达载体构建[J].中国麻业科学,2014,(2):57-63,7.基金项目
中国农业科学院基本科研业务项目 （）
