中国农业科技导报Issue(6)：135-141,7.DOI:10.3969/j.issn.1008-0864.2013.06.20
基于MHC-DAB基因内含子序列的3种虹鳟养殖群体遗传结构分析
Genetic Structure Analysis of Three Cultured Populations of Rainbow Trout(Oncorhynchus mykiss) Based on MHC-DAB Gene Intron Sequence
摘要
Abstract
Oncorhynchus mykiss was cultured all over the world, this paper analyzed the genetic variation of MHC-DAB intron 2 sequences ( 308 bp) in Gansu golden trout, Donaldson rainbow trout and USA Golden trout using PCR-SSCP. 11 different nucleotide sequences(Ⅰ~Ⅺ) were detected from 108 positive clones, a maximum of 4 alleles were amplified from one single individual. Allele Ⅱ,Ⅲ andⅥwere presented in all the 3 populations. Ⅳwas only presented in Donaldson rainbow trout; Ⅶ only presented in Gansu golden trout; Ⅸ, Ⅹ and Ⅺ only presented in Golden trout. In Gansu golden trout, Donaldson rainbow trout and Golden trout, the nucleotide polymorphic sites were 17, 19 and 22. The nucleotide diversities (Pi) were 0.005, 0.008 and 0.013. The average numbers of nucleotide differences ( K ) were 1. 200, 2. 000 and 3. 333, respectively. The genetic distance of Gansu golden trout and Donaldson rainbow trout was the smallest ( 0�231) , while that of Gansu golden trout and Golden trout was the largest (0.539). The genetic differentiation that compared any 2 of the 3 populations all up to the standard of population division (D>0.05). The genetic differentiation index (GST) of the 3 populations was 0.097, indicating that the variation mainly came from the inter-individual of the population.关键词
虹鳟( Oncorhynchus mykiss)/遗传结构/遗传分化/MHCKey words
Oncorhynchus mykiss/genetic structure/genetic differentiation/MHC分类
农业科技引用本文复制引用
杨娟,刘山东,施海娜,胡丹丹,王建福,宋超,张久盘,刘哲..基于MHC-DAB基因内含子序列的3种虹鳟养殖群体遗传结构分析[J].中国农业科技导报,2013,(6):135-141,7.基金项目
甘肃省农业生物技术研究与应用开发项目（ GNSW-2011-21）资助。 （）
