中国农业科学Issue(18)：3743-3749,7.DOI:10.3864/j.issn.0578-1752.2013.18.001
棉花花药全生育期均一化全长cDNA文库的构建和分析
Construction and Analysis of Normalized Full-Length cDNA Library of Upland Cotton Anthers
摘要
Abstract
[Objective]The objective of this study is to understand the anther development and make a firm foundation for sterile mechanism in upland cotton (Gossypium hirsutum L.). [Method]A normalized full-length cDNA library from anthers including the full development stage was firstly constructed and 9 896 high-quality ESTs were sequenced in this study. The bioinformatics analysis and qRT-PCR were performed to deep analyze this cDNA library. [Result]The storage capacity of this cDNA library is 1.2×107 and the average length of the inserted cDNA fragment was dispersed between 1 kb and 3 kb. These high-quality ESTs were further assembled into 6 643 unigenes with 779 bp in average length. Through bioinformatics analysis of these unigenes, many important biological processes were obtained during the anther development and 28 genes related with the biosynthesis of pollen wall were identified during anther development. The qRT-PCR was performed to check the expression pattern of these pollen wall biosynthesis related genes and all of them were high-expressed in early anther development especially in tetrad pollen period. [Conclution]In this study, lots of anther expressed genes were firstly sequenced in upland cotton. Many important processes regulating anther development were obtained and many genes related with pollen wall biosynthesis were obtained.关键词
陆地棉/cDNA文库/花药/花粉壁Key words
Gossypium hirsutum L./cDNA library/anther/pollen wall引用本文复制引用
马建辉,刘记,范术丽,宋美珍,庞朝友,魏恒玲,喻树迅..棉花花药全生育期均一化全长cDNA文库的构建和分析[J].中国农业科学,2013,(18):3743-3749,7.基金项目
国家高技术研究发展计划（2011AA10A102）、国家重点基础研究发展计划 （）
