中国农业科学Issue(18)：3758-3767,10.DOI:10.3864/j.issn.0578-1752.2013.18.003
玉米叶片蛋白对AM菌根接种和（或）砷胁迫的应答
Response of Maize Leaf Proteins Induced/Modulated by AM Mycorrhizal Inoculation and (or) Arsenic Stress
摘要
Abstract
[Objective]In this paper, proteomics were performed to analyze the changes in leaf proteins of maize in response to As additions and mycorrhizal inoculation.[Method]The leaf proteome of maize was analyzed using two-dimensional electrophoresis and mass spectrometry. A targeted proteomic approach was applied to determine the putative identity of some proteins induced/modulated by arsenic (As) and/or mycorrhizal inoculation.[Result]The proteomics aimed at comparing the leaf protein profiles of maize growing in substrate amended with arsenic or unamended. Seven differentially expressed protein spots were identified. Three of them were identified as unknown protein and the remaining four were 2-phosphoglycerate kinase, oxidoreductase, MAP3K delta-1 protein kinase and Os06g0262800. Another comparasion of leaf proteins between plants with arsenic stress and those with As stress plus mycorrhizal inoculation revealed that 22 spots were differentially displayed in response to mycorrhizal inoculation and 11 differentially expressed protein spots were identified. Four of them were identified as unknown protein and the other seven were oxygen-evolving enhancer protein 3-1, putative M protein, SNF1-related protein kinase 2.2, ATP synthase CF1 beta subunit, ATP synthase CF1 alpha subunit, pathogenesis-related protein 10, MAP kinase kinase and MEI1 protein, respectively.[Conclusion] The dry weights of maize plants with As addition were improved by mycorrhizal innoculations, root As concentrations were also decreased significantly. Changes in the amount and types of leaf protein expression in maize plants indicated that mycorrhizal inoculation might simulate the proteins associated with plant growth, nutrient uptake and resistance to produce responses under arsenic stress conditions. Therefore, it’s helpful to improve the resistance of maize plants to arsenic toxicity.关键词
丛枝菌根真菌/玉米/比较蛋白质组学/砷胁迫Key words
abuscular mycorrhizal fungi/Zea mays/comparative proteomics/As stress引用本文复制引用
王真辉,袁坤,杨礼富,张俊伶..玉米叶片蛋白对AM菌根接种和（或）砷胁迫的应答[J].中国农业科学,2013,(18):3758-3767,10.基金项目
国家自然科学基金项目 （）
