中国农业科学Issue(18)：3793-3799,7.DOI:10.3864/j.issn.0578-1752.2013.18.007
斜纹夜蛾受翻译控制肿瘤蛋白基因的克隆及表达
Molecular Cloning and Expression of Translationally Controlled Tumor Protein of Spodoptera litura (Fab.)
摘要
Abstract
[Objective] The objective of this study is to clone the full-length cDNA of TCTP from Spodoptera litura cells, as well as to analyze its sequence.[Method]The full-length cDNA of TCTP was obtained by RACE-PCR. A phylogenetic tree of TCTP protein in different species was constructed. TCTP was cloned in pET-32a (+) and was expressed as fusion protein in Escherichia coli BL21(DE3) cells.[Result]The full-length cDNA of SLTCTP (GenBank accession number:HQ896486.1) was 829 bp with an open reading frame of 519 and encodes 172 amino acids. According to Protparam on-line tools, the deduced protein was predicted to have a computed molecular mass of 19.67 kD. TCTP cloned in pET-32a (+) was expressed as fusion protein in E. coli BL21 (DE3) cells. In order to obtain much target protein of TCTP, optimal culture conditions of pET-32a (+)-TCTP were studied. Induced by 1mmol·L-1 IPTG for 4 h was the best optimized condition for recombinant protein expression. TCTP gene belonged to the TCTP protein family and had a high similarity with other species.[Conclusion]This study has provided a basis of method for further research on the function of SLTCTP and pest control.关键词
斜纹夜蛾/TCTP基因/克隆和表达/序列分析Key words
Spodoptera litura/translationally controlled transcription proteins (TCTP) gene/gene clone and expression/sequence analysis引用本文复制引用
卢梦玲,闫超,毛根林,徐汉虹..斜纹夜蛾受翻译控制肿瘤蛋白基因的克隆及表达[J].中国农业科学,2013,(18):3793-3799,7.基金项目
农业部行业专项（200903052）、广东省科技基础条件建设项目 （）
