中国农业科学Issue(20)：4197-4209,13.DOI:10.3864/j.issn.0578-1752.2013.20.003
基于SSR标记的中国绿豆种质资源遗传多样性研究
Genetic Diversity Research of Mungbean Germplasm Resources by SSR Markers in China
摘要
Abstract
[Objective]The purpose of this research was to investigate the genetic diversity, relationship and differentiation of cultivated mungbean germplasm resources in China, and provide useful information on effective utilization of resources, new genes mining and breeding.[Method]The genetic diversity of 18 mungbean groups from different areas was analyzed by using 40 SSR markers.[Result]A total of 125 alleles were identified with an average of 3.1 alleles per locus. The average of effective number of alleles (NE), Nei's gene diversity (H), polymorphism information content(PIC), expected heterozygosity (He), and Shannon’s information index (I) were 1.8, 0.4233, 0.3497, 0.4241, and 0.6754, respectively. Hebei, Shandong and Anhui provinces had a higher genetic variation than other provinces. The average observed heterozygosity (Ho) was 0.1001 and the average of inbreeding coefficient among population(Fis) was 0.6759, indicating that a certain degree of inbreeding existed in Chinese mungbean germplasm. The gene flow (Nm=0.6936) and the average genetic differentiation (Fst=0.2649) also showed a high genetic variation within Chinese mungbean. A total of 272 individuals were divided into 2 groups and 18 populations were divided into 3 groups based on Popgene software. The genetic distances between groups were agreeable with their geographical origin.[Conclusion]There was a high genetic diversity level within Chinese mungbean germplasm. Geographical environment and ecological factors affect the genetic differentiation among different populations.关键词
绿豆/SSR/遗传多样性/亲缘关系Key words
mungbean/SSR/genetic diversity/genetic relationship引用本文复制引用
刘岩,程须珍,王丽侠,王素华,白鹏,吴传书..基于SSR标记的中国绿豆种质资源遗传多样性研究[J].中国农业科学,2013,(20):4197-4209,13.基金项目
国家食用豆产业技术体系建设项目（CARS-09）、食用豆行业科技专项（nyhyzx07-017）、作物种质资源保护 （）
