中国农业科学Issue(22)：4665-4678,14.DOI:10.3864/j.issn.0578-1752.2013.22.004
花后干湿交替灌溉对水稻强、弱势粒蛋白质表达的影响
Effect of Post-Anthesis Alternate Wetting and Drying Irrigation on Protein Expressions in Superior and Inferior Spikelets of Rice
摘要
Abstract
[Objective]This study aimed to understand the mechanism underlying the effect of alternate wetting and drying irrigation on grain filling of rice. [Method]Two rice cultivars, Liangyoupeijiu (two-line indica hybrids) and Yangjing 4038 (japonica), were grown in cement tanks. Three irrigation regimes, conventional irrigation (CI), alternate wetting and moderate drying irrigation (WMD) and alternate wetting and severe drying irrigation (WSD), were imposed from full heading to maturity. The expressions of the proteins in superior and inferior spikelets were investigated using the method of two-dimensional gel electrophoresis.[Result]No significant differences in grain-filling rate and grain weight were observed for the earlier-flowering superior spikelets among the three irrigation regimes. For the later-flowering inferior spikelets, however, their grain-filling rate and grain weight were significantly increased under the WMD, but significantly reduced under the WSD when compared with those under the CI. Both WMD and WSD showed no significant impact on the protein expressions in superior spikelets. However, the WMD enhanced expressions of many proteins, such as orthophosphate dikinase, glyceraldehyde-3-phosphatedehydrogenase, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, S-adenosylmethionine synthetase, glyoxalase I and manganese superoxide dismutase in inferior spikelets, whereas the WSD inhibited expressions of these proteins. The WSD also up-regulated expressions of some proteins, such as the proteins related to the inhibition of signal transduction and energy metabolism.The two cultivars exhibited the same tendencies. [Conclusion] Expression differences of the proteins related to grain filling in inferior spikelets account for their increases or decreases in grain-filling rate and grain weight under the WMD or WSD.关键词
水稻/干湿交替灌溉/弱势粒/灌浆/蛋白质表达Key words
rice/alternate wetting and drying irrigation/inferior spikelets/grain filling/protein expression引用本文复制引用
陈婷婷,褚光,华小龙,潘燕,王志琴,杨建昌..花后干湿交替灌溉对水稻强、弱势粒蛋白质表达的影响[J].中国农业科学,2013,(22):4665-4678,14.基金项目
国家自然科学基金重大国际合作交流项目（31061140457）、国家自然科学基金项目（31271641，31071360）、国家公益性行业（农业）科研专项（201103003，201203079）、国家“十二五”科技支撑计划项目（2011BAD16B14）、江苏高校优势学科建设工程专项、江苏省普通高校研究生科研创新计划项目 （）
