中国农业科学Issue(22)：4698-4706,9.DOI:10.3864/j.issn.0578-1752.2013.22.007
菜粕生物熏蒸防控辣椒疫病
Effects of Biofumigation with Rapeseed Meal on Disease Control of Phytophthora Blight of Chilli Pepper
摘要
Abstract
[Objective]The objective of this study is to research the effects of hydrolysis product of rapeseed meal (RSM) on growth inhibition of Phytophthora capsici and on disease control of Phytophthora blight of chilli pepper cultivation in greenhouse.[Method] Inhibition effect of hydrolysis product of RSM on mycelium and zoospore was studied by in vitro test in dishes and imitated biofumigation in container, and disease control effects of biofumigation with RSM2 on Phytophthora blight of chilli pepper in pot and field were also carried out. [Result] Volatiles and non-volatiles released from RSM hydrolysis inhibited mycelium growth and zoospore germination in various degrees, and the inhibition effect of RSM2 was better than that of RSM1. The inhibition effect of volatiles produced from RSM hydrolysis on zoospore was stronger than on mycelium of P. capsici while non-volatiles displayed stronger inhibition effect on mycelium than on zoospore. The inhibition effect of volatiles and non-volatiles showed no significant difference on mycelium but on zoospore of P. capsici. Soil fumigation in sealed container was carried out in laboratory at different application rates of RSM and the results showed that volatiles released from RSM hydrolysis killed mycelium of P. capsici when RSM was added to soil at the rate of 0.2%(w/w). In a pot experiment, real-time PCR was adopted to assess the population of P. capsici. The results showed that the efficacy of disease control of RSM treatment for Phytophthora blight was 100%, the same as dazomet treatment, although P. capsici exsisted in soil at density of 36 zoospore/g soil. RSM2 treatment not only increased the population of fungi and actinomycetes but also increased the total number and diversity of soil microbe. The plot experiment conducted in greenhouse showed that the average value of control efficacy for Phytophthora blight was 82% and the yield of chilli pepper increased with 16.4%in two continuous cropping seasons. The effect of RSM treatment in chilli pepper production in protected field was better than dazomet treatment. [Conclusion]RSM2 showed great inhibition effect on growth of P. capsici. The treatment of biofumigation with RSM2 suppressed disease incidence of Phytophthora blight in field efficiently and increased yield of chilli pepper.关键词
生物熏蒸/菜粕/辣椒疫霉/土壤微生物/辣椒疫病Key words
biofumigation/rapeseed meal/Phytophthora capsici/soil microbe/Phytophthora blight of chilli pepper引用本文复制引用
马艳,胡安忆,杨豪,常志州,徐跃定,张建英..菜粕生物熏蒸防控辣椒疫病[J].中国农业科学,2013,(22):4698-4706,9.基金项目
农业部公益性行业计划项目（201103004-9）、江苏省农业科技自主创新基金项目（cx（11）2020） （）
