中国农业科学Issue(23)：5003-5011,9.DOI:10.3864/j.issn.0578-1752.2013.23.017
应用FISH-AFLP技术分析平欧杂种榛主栽品种的遗传关系
Analysis of the Genetic Relationship of the Main Cultivars of Ping’ou Hybrid Hazelnut (C. heterophylla×C. avellana) by FISH-AFLP Markers
摘要
Abstract
[Objective]The objective of this study is to establish FISH-AFLP analysis system for Ping’ou hybrid hazelnut and analyze the genetic relationship of the main cultivars.[Method]Ten main cultivars, such as Dawei, were selected as materials. High-quality genomic DNA was extracted with improved CTAB method, and the purified DNA samples were digested with PstⅠ/MseⅠ. After ligation reaction, the samples were used to perform FISH-AFLP detection. The information of all bands was converted into“0-1”matrix. NTSYS pc 2.11F and Popgene 1.32 software were used to conduct the data analysis and plotting.[Result]The results showed that:15 PstⅠ/MseⅠ primer pairs were selected from 64 combinations with high polymorphism, strong signals and clear bands. A total of 1 739 bands were obtained. The average percentage of polymorphic bands was 97.94%. The similarity coefficient of the main cultivars varied from 0.7556 to 0.8543. All the samples were divided into 4 groups when the threshold value was 0.8398. Each in cutivars of Yuzhui (84-310), Liaozhen No. 4 (85-41) and Ping’ou 69 (84-69) formed an independent group alone and the others constituted the fourth group. The effective number of alleles, Nei’s gene diversity and Shannon’s information index of the main cultivars were 1.3921, 0.2482 and 0.3957, respectively, indicating high genetic diversity among Ping’ou hybrid hazelnut. The unique bands obtained in the experiments could be used in the rapid identification of the main cultivars of Ping’ou hybrid hazelnut. [Conclusion] Due to the complex selecting group, Ping’ou hybrid hazelnut is a high polymorphic population and the genetic relationships of the main cultivars are complex. The FISH-AFLP analysis system, PstⅠ/MseⅠprimer pairs and all the genetic parameters obtained in this research can offer reference for the relative researches in Corylus.关键词
平欧杂种榛/FISH-AFLP/指纹图谱/亲缘关系/遗传多样性Key words
Ping’ou hybrid hazelnut (C. heterophylla×C. avellana)/FISH-AFLP markers/fingerprinting/genetic relationship/genetic diversity引用本文复制引用
马庆华,陈新,赵天田,刘庆忠,王贵禧..应用FISH-AFLP技术分析平欧杂种榛主栽品种的遗传关系[J].中国农业科学,2013,(23):5003-5011,9.基金项目
国家林业局林业公益性行业科研专项（201304710）、中央级公益性科研院所基本科研业务费专项资金（RIF2012-11）、山东省农业良种工程项目-鲁科农字 （）
