中国农业科学Issue(24)：5173-5179,7.DOI:10.3864/j.issn.0578-1752.2013.24.011
白菜黑斑病抗性鉴定及QTL定位
Evaluation and QTL Mapping for Resistance to Black Spot (Alternaria brassicicola) in Brassica rapa
摘要
Abstract
[Objective]The aim of this study is to evaluate Brassica rapa resistance to black spot disease (Alternaria brassicicola), and map QTL related to this trait. The result will provide theoreticalbasis for genetic improvement on resistance to black spot disease in B. rapa crops.[Method]Conidia of A. brassicicola was sprayed to 30-day-old B. rapa seedlings or detached leaves. Disease index was scored after seedlings or detached leaves being kept in dark and wet for three to six days to determine the number of days for inoculation to distinguish the difference in resistance among B. rapa accessions. Using the optimized method, 70 B. rapa accessions were screened for their resistance to black spot disease. Furthermore, a population with 122 recombinant inbred lines (RILs) was used to map QTL for black spot resistance in B. rapa.[Result]It was found that inoculation of detached leaves for five days could well distinguish the resistance to black spot disease in B. rapa. Fourteen from 70 B. rapa accessions were identified as resistant to black spot disease. Moreover, we mapped two QTLs related to resistance to black spot in B. rapa, one of which was identified in both two experiments. [Conclusion]The study showed that different levels of resistance to black spot disease were clearly distinguished when the detached 3rd/4th true leaves of B. rapa being inoculated with A. brassicicola conidia in dark and wet for 5 days. No accession with high resistance to black spot disease was identified in this study. Two QTLs related to resistance to black spot were mapped on chromosomes A05 and A06, respectively.关键词
白菜/黑斑病/抗性鉴定/离体叶/QTL定位Key words
Brassica rapa/black spot/identification for resistance/detached leaf/mapping QTL引用本文复制引用
陈莹,柳李旺,谢学文,孟霖,刘博,王晓武,李宝聚,武剑..白菜黑斑病抗性鉴定及QTL定位[J].中国农业科学,2013,(24):5173-5179,7.基金项目
中央级公益性科研院所基本科研业务费专项（1610032011011）、农业部园艺作物生物学与种质创制重点实验室资助 （）
