中国农业科学Issue(1)：111-121,11.DOI:10.3864/j.issn.0578-1752.2014.01.012
中国50个甘蓝代表品种EST-SSR指纹图谱的构建
EST-SSR Fingerprinting of Fifty Cabbage Representative Varieties from China
摘要
Abstract
[Objective]In this study, the method of cabbage DNA fingerprint was drawn, and fifty cabbage representative varieties from China were fingerprinted with EST-SSR primers to provide reference for variety distinctness, authenticity, and purity identification. [Method]First, EST-SSR primers were screened by using the technologies of 6% denaturing polyacrylamide gel electrophoresis and six cabbage varieties that come from different ecogeography. The length of amplified fragments was detected on DNA Analyzer platform using four fluorescent-labels (TAMRA, HEX, ROX and 6-FAM) in 5′end of forward primer, and then defined the reference variety for every alleles. Total twenty core primers were used to establish fifty cabbage varieties SSR fingerprinting, and for ‘Zhonggan 21’ variety identification. [Result] Six cabbage varieties of different resources were used to screen 978 EST-SSR primers, out of 128 polymorphic primers were obtained according to the PCR bands stability, high polymorphism information content (PIC), easy discrimination of different alleles and even distribution of molecular markers on each chromosome, and 20 pairs of primers were selected to detect a total of 58 alleles at 20 loci, with 2.22 loci per chromosome and 2.9 alleles per locus on average. The PIC values varied among the primers ranging from 0.34 to 0.76. The length of amplified fragment varied in the range of 143-296 bp. The maximum number of alleles for each primer pairs of BoE607 and BoE723 was five. Fingerprinting database of 50 cabbage representative varieties from China was established with 20 pairs of core primers. The authenticity of‘Zhonggan 21’ was identified by artificial simulated population and these results were identical with that made from field investigation.[Conclusion]Twenty pairs of core primers were selected and used to establish DNA fingerprint database of 50 cabbage representative varieties from China, and the authenticity of‘Zhonggan 21’ was identified by artificial simulated population and the accuracy was 100%.关键词
甘蓝/SSR标记/指纹图谱/品种鉴定Key words
cabbage/SSR marker/fingerprinting/varieties identification引用本文复制引用
王庆彪,张扬勇,庄木,杨丽梅,刘玉梅,吕红豪,方智远..中国50个甘蓝代表品种EST-SSR指纹图谱的构建[J].中国农业科学,2014,(1):111-121,11.基金项目
公益性行业（农业）科研专项经费项目（200903008）、国家自然科学基金（31272180）、中央级公益性科研院所基本科研业务费专项（ICSCAAS2010-11）、国家“863”计划（2012AA100101）、农业部园艺作物遗传改良重点开放实验室项目 （）
