中国蔬菜Issue(18)：35-41,7.
甜瓜果实相关性状QTL分析
QTL Analysis of Fruits Related Traits in Melon(Cucumis melo L.)
摘要
Abstract
A recombinant inbred lines(RILs)mapping population of melon(Cucumis melo L.) comprising 124 F6:7 families was developed from crossing the thick-skin netted melon line WI998 as female parent and thin-skin melon line 3-2-2 as male parent through single-seed descent. This RILs population was used to construct a linkage map with microsatellite(simple sequence repeat, SSR)markers.Screening from 608 SSR markers 150 pairs of primers(24.67%)were detected to be polymorplic between the 2 parental lines.This map contained 17 linkage groups spanning 1 246.67 cM with a mean marker interval of 9.59 cM.Composite interval mapping method was used to detect QTLs of traits involved in melon fruit:fruit weight(Fw),fruit firmness(Ff),fruit length(Fl),flesh thickness(Ft)and fruit shape index(Fsi).Total 15 QTLs of fruit were detected and mapped on linkage groups 1,4,5,6,8,10,13,14,15,17,respectively. Among them, 8 QTLs had contribution rate over 10%. QTL Ff13.2 located at the 13th linkage group had the larg-est contribution rate 26.45%.Marker ECM87 was located in the same site with Fsi8.2,and marker CM33 was close linkage with Fsi15.1,the genetic distance is 0.6 cM.关键词
甜瓜/SSR标记/遗传图谱/果实/QTLKey words
Cucumis melo L./SSR marker/Genetic map/Fruit/Quantitative trait locus(QTL)分类
农业科技引用本文复制引用
张雪娇,高鹏,栾非时..甜瓜果实相关性状QTL分析[J].中国蔬菜,2013,(18):35-41,7.基金项目
国家西甜瓜产业技术体系分子育种岗位项目（CARS-26-02），农业部“948”计划项目 （）
