中国蔬菜Issue(20)：24-31,8.
结球甘蓝雌蕊调控转录因子SPT基因的克隆与序列分析
Moclecular Cloning and Sequence Analysis of SPT Gene Transcription Factor Regulation of Pistil from Cabbage
摘要
Abstract
Taking cabbage(Brassica oleracea L.)E1 as materials,We extracted total RNA from capullo,reverse transcribed cDNA. According to SPT gene sequence of Arabidopsis,primers were designed and 1 085 bp SPT gene with 1 062 bp open reading frame(ORF)was cloned by ho-mology cloning techniques. The deduced BoSPT protein contained 353 amino acids,with a molecu-lar weight of 37.67 kD and pI of 6.83. After double enzyme of EcoRI and KpnI restriction enzymes, and then construct the recombinant plasmids pET43.1a-BoSPT. After transformation to E. coli Ro-setta(DE3),the expression of recombinant proteins were detected via SDS-PAGE. The structural analysis of BoSPT though Smart-embl showed that it contained bHLH family domain,which located at the position of 173-221 amino acid residues,The phylogenetic tree indicated that the BoSPT had close genetic relationship with AtSPT and AlSPT. Prokaryotic expression showed that the molecular mass of BoSPT protein was purified.关键词
结球甘蓝/雌蕊/SPT/基因克隆/序列分析Key words
Cabbage/Pistil/SPT/Moclecular cloning/Sequence analysis分类
农业科技引用本文复制引用
杨朴丽,许俊强,刘智宇,王志敏,张丹华,汤青林,宋明..结球甘蓝雌蕊调控转录因子SPT基因的克隆与序列分析[J].中国蔬菜,2013,(20):24-31,8.基金项目
国家重点基础研究发展计划项目（2012CB113900），国家自然科学基金项目（31000908），重庆市自然科学基金项目（2011BA1002），中央高校基本科研业务费专项（XDJK2012B020），国家大学生创新项目 （）
