中国水产科学Issue(6)：1123-1131,9.DOI:10.3724/SP.J.1118.2013.01123
半滑舌鳎Dorsalin-1-like基因的克隆与表达
Molecular cloning and expression analysis of Dorsalin-1-like gene in the tongue sole (Cynoglossus semilaevis)
摘要
Abstract
As a member of the bone morphogenetic protein (BMP) family, Dorsalin-1 plays an important role in regulating the development of the nervous system. To date, studies involving the vetebrate Dorsalin-1 has thus far only rarely been reported. In this study, molecular cloning and expression analysis of Dorsalin-1 gene were performed in the tongue sole (Cynoglossus semilaevis). As a result, the Dorsalin-1-like cDNA sequence is 1 961 bp, which contains a 5’-untranslated region (UTR) of 153 bp, an open reading frame (ORF) of 1 260 bp encoding a protein of 419 amino acids, and a 3’-untranslated region (UTR) of 548 bp. The homology and phylogenetic analysis revealed that the deduced amino acid sequence of C. semilaevis Dorsalin-1-like gene exhibited the highest identity with those of Takifugu rubripes and Oreochromis niloticus, and the fish Dorsalin-1 formed a single lineage from the other vertebrates. The tissue expression distribution pattern of Dorsalin-1-like gene was examined in the 13 tissues of female and male adults. The results showed that the Dorsalin-1-like mRNA was predominantly expressed in heart, and less expressed in the liver and spinal cord. Finally, the Dorsalin-1-like mRNA levels at early developmental stages were also detected at egg, larva, juvenile and fingerling stages. The highest level was observed in the embryonic stage (egg stage) and early-stage larvae (larva stage). These findings suggest that Dorsalin-1 may play an important role in regulating early development of the egg and larva stages, and maybe involved in the heart and skeletal development through the regulation of the nervous system.关键词
半滑舌鳎/Dorsalin-1-like/mRNA表达/早期发育/骨骼发育/神经系统Key words
Cynoglossus semilaevis/Dorsalin-1-like/mRNA expression/early developmente/skeleton growth/nervous system分类
农业科技引用本文复制引用
马骞,庄志猛,柳淑芳,唐启升..半滑舌鳎Dorsalin-1-like基因的克隆与表达[J].中国水产科学,2013,(6):1123-1131,9.基金项目
国家自然科学基金项目(31201981) （31201981）
中国水产科学研究院黄海水产研究所级基本科研业务费资助项目(20603022012032) （20603022012032）
