中国水产科学Issue(6)：1132-1138,7.DOI:10.3724/SP.J.1118.2013.01132
半滑舌鳎Dmrt1蛋白表达、纯化及功能
Protein expression, purification, and elementary function of Dmrt1 in half-smooth tongue sole (Cynoglossus semilaevis)
摘要
Abstract
We evaluated the role of Dmrt1 in regulating sex determination and differentiation in half-smooth tongue sole (Cynoglossus semilaevis). The recombinant protein of C. semilaevis Dmrt1 was expressed by the prokaryotic expression system (Pet-32a) and the protein (Pet-32-dmrt1) was subsequently purified by immobilized-metal affinity chromatography. We measured gene expression after injection of the recombinant protein Pet-32-Dmrt1 into the ovary. The expression of CYP19A and Foxl2 decreased significantly between 6 and 24 h after injection and the mRNA transcription level of Sox9aa was significantly increased between 6 and 24 h. However, the expression of all three genes expression returned to normal 48 h after injection. Our results indicate that Dmrt1 recombinant protein has biological activity, and plays a role in sex differentiation by regulating sex related gene expression.关键词
半滑舌鳎/Dmrt1/蛋白表达/功能/性别决定Key words
Cynoglossus semilaevis/Dmrt1/protein expression/elementary function分类
生物科学引用本文复制引用
胡乔木,王凯琳,陈松林..半滑舌鳎Dmrt1蛋白表达、纯化及功能[J].中国水产科学,2013,(6):1132-1138,7.基金项目
国家863计划项目(2012AA092203) （2012AA092203）
国家自然科学基金项目(31130057,31072202) （31130057,31072202）
山东省泰山学者工程专项资助 （）
