中国水产科学Issue(6)：1197-1203,7.DOI:10.3724/SP.J.1118.2013.01197
仿刺参纵肌带再生的形态学和组织学
Morphology and histology of longitudinal muscle band regeneration in Apostichopus japonicus
摘要
Abstract
We documented the regeneration of longitudinal muscle bands in the sea cucumber, Apostichopus ja-ponicus. The longitudinal muscle bands at the rear of the abdominal cavity were severed with scissors. The sea cucumbers were then reared in sea water containing antibiotics (100 IU/mL penicillin and 100μg/mL streptomy-cin), and the structural changes of the regenerated longitudinal muscle bands were documented using morpho-logical and histological methods. Morphological analysis revealed a gap of 0.5-1 cm around the broken muscle bands caused by contraction of the longitudinal muscle after surgery. Ivory and flocculent tissue (muscle precur-sors) were observed at the trauma site 15 d after the operation. The muscle precursor thickened 30-45 d after in-jury and the ends of the severed muscle were reconnected. The muscle precursor gradually transformed into mus-cle bands that were half the thickness of the normal longitudinal muscle 60-90 d after surgery. The regenerated longitudinal muscle bands grew further, and were only slightly smaller in diameter 110-130 d after surgery. The longitudinal muscle bands were completely regenerated 150 d after surgery, and there was no significant difference from undamaged muscle fibers. Histological analysis revealed that the muscle precursor was composed of connec-tive tissue and individual muscle fibers 15 d after surgery. At 30-45 d, the number of new muscle cells increased significantly, and there was evidence of“bridged’ connections between thickening muscle precursors and the body wall. At 60-90 d, the thickening muscle precursor was largely replaced by muscle fibers, and could be classified as longitudinal muscle bands. Concurrently, the number of“bridged’ connections increased. At 110-130 d, the num-ber of new muscle fibers increased significantly and the number of“bridged”connections decreased. At 150 d, the longitudinal muscle bands had regenerated completely and the “bridged” connections had disappeared. Our ob-servations suggest that the longitudinal muscle bands have a strong ability for regeneration and the new muscle cells are derived from the connective tissue cells in the body wall and the coelomic epithelium.关键词
仿刺参/纵肌带/再生/形态学/组织学Key words
sea cucumber/Apostichopus japonicus/longitudinal muscle bands/regeneration/morphology/histology分类
农业科技引用本文复制引用
李霞,赵丽娜,秦艳杰..仿刺参纵肌带再生的形态学和组织学[J].中国水产科学,2013,(6):1197-1203,7.基金项目
国家自然科学基金资助项目(30371099) （30371099）
辽宁省自然科学基金资助项目(20052139) （20052139）
辽宁省创新团队项目(2007T015) （2007T015）
