中国水产科学Issue(6)：1219-1224,6.DOI:10.3724/SP.J.1118.2013.01219
不同技术处理的钝齿短浆蟹淋巴液中和麻痹性贝毒毒性的效果
Effect of treatment methods of hemolymph from shore crab Thalamita crenata on its paralytic shellfish poisoning toxicity neutralization
摘要
Abstract
Hemolymph from shore crab, Thalamita crenata, is able to neutralize the toxicity of paralytic shellfish poi-soning (PSP) toxins. This information offers a groundwork on investigating the detoxifcation of the PSP. Unfortunately, whether or not the treatment methods of the hemolymph will affect the PSP toxicity neutralization is still unclear. In this study, the effects of anticoagulants (EDTA, sodium oxalate, sodium citrate and heparin sodium), extracting methods (syringe needling extraction and tissue homogenizing extraction) and freezing storage condition (with or without su-crose) on PSP toxicity neutralization of the hemolymph were investigated through the mouse assay. The results showed that the mice intraperitoneal injection with the PSP mixed with EDTA-hemolymph were survival, while the mice’s death times of hemolymph groups mixed with sodium oxalate, sodium citrate and heparin sodium were (27.86 ± 2.66) min, (28.89 ± 0.59) min and (14.08 ± 0.6) min, which to be 5.4, 5.3 and 2.6 times to that of corresponding control, respec-tively. Low EDTA concentration was efficacious in anticoagulation of the hemolymph and preserving a good PSP neutralization, which seemed to be repressed with in presence of excessive EDTA. Hemolymph extracted by syringe needle showed higher PSP neutralization than that of extracted by tissue homogenization. While the EDTA concentra-tion was at 5 mmol/L in 20 mmol/L phosphate buffer (pH 8.0), 67%of the mice for the hemolymph group extracted by syringe needle died at more than 30 minutes. By comparison, the mice’s death time for hemolymph group extracted by tissue homogenization ranged at 8.1−24.0 min. Moreover, sucrose could protect the hemolymph against from freeze denaturation. After freezing for 2 weeks, the PSP neutralization of the hemolymph with sucrose remained unchanged, while that of the hemolymph without sucrose decreased. These results indicate that the PSP neutralization of hemolymph was remarkably effected by anticoagulants, extraction methods and freezing storage condition.关键词
钝齿短浆蟹/麻痹性贝毒(PSP)/淋巴液/毒性中和效果Key words
Thalamita crenata/paralytic shellfish poisoning (PSP) toxins/hemolymph/PSP toxicity neutralization分类
生物科学引用本文复制引用
陈晓,姜沛宏,林华娟..不同技术处理的钝齿短浆蟹淋巴液中和麻痹性贝毒毒性的效果[J].中国水产科学,2013,(6):1219-1224,6.基金项目
广东省国际合作项目(粤科规划字[2012]98号) （粤科规划字[2012]98号）
农业部现代农业产业技术体系建设专项(贝类,47) （贝类,47）
