中国水产科学Issue(1)：75-83,9.DOI:10.3724/SP.J.1118.2014.00075
生物絮团培养过程中养殖水体水质因子及原核与真核微生物的动态变化
Dynamic changes of water quality factors and composition of pro-karyotic and eukaryotic microorganisms during culturing of bio-floc
摘要
Abstract
A 30-day experiment was conducted to investigate the effectiveness of bio-floc technology for main-taining good water quality in Ctenopharyngodon idellus with average weight of (121.61±14.23) g farming ponds. The total suspended solids(TSS), total nitrogen (TN) and alkalinity (CaCO3) were determined every 3 days and microbial communities of the bio-floc were analyzed using the PCR-DGGE (PCR-denaturing gradient gel elec-trophoresis) every 5 days, respectively. The results showed that water TSS concentration reached a peak value of (419.67±11.5) mg/L on the 9th day, which was significantly (P<0.05) higher than that of the other time points. From the 15th day on, the TSS remained at a relatively stable level of 244.67 mg/L. The TN ranged from 6.65 to 11.15 mg/L, and it reached the peak value of (10.11±1.05) mg/L on the 9th day. There was no significant differ-ence in TN between water after the 12th day and water at the beginning of the farming trial (P>0.05). Alkalinity in the farming system ranged from 136.68 to 239.20 mg(CaCO3)/L, which reached maximum peak value of (238.22±5.44) mg (CaCO3)/L on the 6th day, after which it decreased gradually and then remained stable. Bio-floc had a crude protein content of 30%dry weight. The acquired sequences of 20 bands in 16S rDNA DGGE gel and six bands in 18S rDNA DGGE gel were analyzed by BLAST searches against the NCBI GenBank database. The main prokaryotic microorganisms represented by the 20 main bands in 16S rDNA DGGE gel were Alphaproteo-bacteria, Betaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Actinobacteria, and Bacteroidetes. Alca-ligenaceae throughout the process of formation of the bio-floc, and was the dominant bacteria on the 5th, 10th, and 15th days. The existence of Alcaligenaceae had some influence on the increase in water alkalinity during this pe-riod. We also found that bands 7 and12 represented Cyanobacteria, which appeared on the 0th, 5th and 10th days;they disappeared in the late culture period, which indicated that the application of bio-floc technology can control algal growth to some extent. The main Eukaryotic microorganisms represented by the six main bands in 18S rDNA DGGE gel were Loxophyllum sp., Nitzschia amphibian, Goniomonas sp., and Cryptomonadaceae. Nitzschia am-phibian belongs to the diatoms, and was present from the 1stto 15th days of bio-floc culture. A larger number of Loxophyllum sp. existed at the later stage of the bio-floc culture. The results demonstrated that the bio-floc system achieved stable operation state after 15 days. Bio-floc could regulate the water quality and the distribution of bac-teria and algae effectively;it could also maintain the balance and development of the whole aquaculture system.关键词
生物絮团/PCR-DGGE/草鱼/原核微生物/真核微生物/总氮Key words
Bio-floc/PCR-DGGE/Ctenopharyngodon idellus/prokaryotic microorganisms/eukaryotic microor-ganisms/total nitrogen分类
农业科技引用本文复制引用
夏耘,邱立疆,郁二蒙,谢骏,王广军,余德光,吉红..生物絮团培养过程中养殖水体水质因子及原核与真核微生物的动态变化[J].中国水产科学,2014,(1):75-83,9.基金项目
农业部公益性行业科研专项(201203083) （201203083）
现代农业产业技术体系建设专项(CARS-46-17) （CARS-46-17）
广东省海洋渔业科技推广专项(B201201A05) （B201201A05）
“十二五”农村领域国家科技计划课题(2012BAD25B01) （2012BAD25B01）
