中国水产科学Issue(1)：161-168,8.DOI:10.3724/SP.J.1118.2014.00161
铜对刀鲚幼鱼的急性毒性及对肝抗氧化酶活性与组织结构的影响
Acute effects of copper on survival of fingerlings, antioxidant enzyme activities in liver and structure of gill and liver of Coilia nasus
摘要
Abstract
We evaluated the acute toxicity of copper on the survival rate of Coilia nasus fingerlings and on antioxidant enzyme (Superoxide dismutase, SOD;Catalase, CAT) activity changes in fish liver. The histology of experimental fish gills and liver were also analyzed. Fish were exposed to copper sulfate (CuSO4) solution with Cu2+concentrations of 0(control), 0.3, 0.54, 0.96, 1.68 and 3 mg/L. The 50%lethal concentration (LC50) values of copper on C. nasus were 1.087, 1.062, 1.042 and 0.9 67 mg/L at 24, 48, 72 and 96 h, respectively. The safe concentration of copper for this spe-cies at the fingerling stage was calculated as 0.009 67mg/L. The activities of CAT and SOD were significantly enhanced in liver cells after the fish were exposed in CuSO4 for 12 and 24 h compared with those in the control group (P<0.05). Forty-eight hours later, the activities of CAT started to decline, while the activities of SOD did not decrease until 72 h. Overall, the CAT (after 48 h) and SOD (after 72 h) in the liver cells of fish exposed to copper sulfate exhibited lower activities than those in the control group. The changes of CAT activities after 48 h positively correlated with Cu2+concentration. Our results indicated that 1.68 mg/L Cu2+was toxic to Coilia nasus fingerlings, resulting in swollen and denatured epithelium and deformed lamellae of the gills. The hepatic ultrastructural observation sampled from the fish exposed to 3 mg/L Cu2+showed significant hypertrophy of liver cells; the mitochondria exhibited vacuolization;vacuoles and lipid droplets occurred in cytoplasm. Copper appeared to induce fatty liver syndrome. We concluded that determining antioxidant enzyme (SOD and CAT) activities and histopathological analysis are important approaches to evaluate the safe concentration of copper for C. nasus fingerlings.关键词
刀鲚幼鱼/铜/安全浓度/抗氧化酶/组织病理Key words
Coilia nasus/fingerling/copper/safe concentration/antioxidant enzyme/histopathology分类
资源环境引用本文复制引用
聂志娟,徐钢春,张守领,徐跑,顾若波..铜对刀鲚幼鱼的急性毒性及对肝抗氧化酶活性与组织结构的影响[J].中国水产科学,2014,(1):161-168,8.基金项目
农业部公益性行业科研专项(201203065) （201203065）
国家科技支撑计划项目(2012BAD26B05) （2012BAD26B05）
江苏省科技支撑计划(农业)项目(BE2011411) （农业）
江苏省水产三新工程重大专项(DZ2012-1) （DZ2012-1）
