中国水产科学Issue(1)：197-202,6.DOI:10.3724/SP.J.1118.2014.00197
牙鲆有丝分裂雌核发育二倍体的制备
Production of mitotic gynogenetic diploid Japanese flounder, Parali-chthys olivaceus
摘要
Abstract
Mitotic gynogenetic diploid Japanese flounder, Paralichthys olivaceus were produced by activating eggs with UV irradiated sperm of red sea bream (Pagrus major), followed by hydrostatic pressure treatment to block the first mitotic division. The initiation time, duration time and pressure value of hydrostatic pressure were opti-mized in this study. The results showed that the treatment with hydrostatic pressure (650 kg/cm2) for 6 min starting 60 min after fertilization was the optimal parameters combination. Under this condition the fertilization rate and hatching rate at this scenario were 67.80%and 54.23%, respectively, which were significantly higher than that of all the other treatment groups(P<0.05). The embryo not treated by hydrostatic pressure (haploid) were identified via flow cytometer, and its relative DNA content was about half that of normal diploid. The mitotic gynogenetic embryo had the same DNA relative content with normal diploid. The analysis results showed that mitotic gynoge-netic diploids could be successfully prepared.关键词
牙鲆/有丝分裂/雌核发育/静水压/优化Key words
Japanese flounder/mitosis/gynogenesis/hydrostatic pressure/optimization分类
生物科学引用本文复制引用
刘永新,刘海金,薛玲玲,王玉芬,张晓彦,司飞,孙朝徽,王桂兴..牙鲆有丝分裂雌核发育二倍体的制备[J].中国水产科学,2014,(1):197-202,6.基金项目
国家现代产业技术体系资助项目(nycytx-50-G02) （nycytx-50-G02）
国家科技支撑计划项目(2012BAD26B01) （2012BAD26B01）
公益性行业(农业)科研专项经费项目(200903046-07) （农业）
