中国水产科学Issue(2)：214-224,11.DOI:10.3724/SP.J.1118.2014.00214
高温诱导对性成熟尼罗罗非鱼性腺中性别分化相关基因表达的影响
Differential expression analysis of genes involved in high-tempera-ture-induced sex differentiation in the gonads of mature Nile tilapia (Oreochromis niloticus)
摘要
Abstract
In Nile tilapia, the mRNA expression level of various sex differentiation-related genes can be affected by high-temperature induction during the critical period of sexual differentiation, triggering sex-reversal from female to physiological male. However, it is unknown whether the effect of high temperature on Nile tilapia (Oreochromis niloti-cus) sex differentiation-related genes is period-specific. In this study, the expression of eight sex differentiation-related genes, four heat shock protein genes and two methyltransferase genes were systematically analyzed in 8-month-old Nile tilapia under high-temperature (36℃) treatment by quantitative real-time RT-PCR techniques. The results showed that the expression levels of Cyp19a1a, ERα and ERβ, which are ovary development-related genes, were down-regulated (P<0.05), but the expression levels of Cyp11b2 and Dmrt1, which are testis development-related genes, were not sig-nificantly changed (P>0.05) under high-temperature treatment. The results differed from those for high-temperature treatment of Nile tilapia during the critical period of sexual differentiation. The expression level of Hsp70 was signifi-cantly up-regulated, while the expression level of DNAJB1 was significantly down-regulated. The expression levels of Hsp27and Hsp90 were not significantly changed in the gonads of females and males under high-temperature induction. Furthermore, it was also found that high temperature significantly up-regulated the expression level of Dnmt1 and Dnmt3a in the female gonad.关键词
尼罗罗非鱼/高温诱导/性别分化相关基因/热激蛋白基因/甲基转移酶基因Key words
Nile tilapia/high-temperature induction/sex differentiation-related gene/heat shock protein gene/methyltransferase gene分类
农业科技引用本文复制引用
李春歌,赵燕,陈红菊,付佩胜,王慧,季相山..高温诱导对性成熟尼罗罗非鱼性腺中性别分化相关基因表达的影响[J].中国水产科学,2014,(2):214-224,11.基金项目
中国博士后科学基金面上项目(20100481295) （20100481295）
山东省青年自然科学基金资助项目(ZR2009DQ021) （ZR2009DQ021）
